Recombinant Human NDST1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5967-ST

R&D Systems, part of Bio-Techne
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5967-ST-010
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Key Product Details

Source

CHO

Accession #

P52848

Conjugate

Unconjugated

Applications

Enzyme Activity

View all N-Deacetylase/N-Sulfotransferase 1/NDST1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human N-Deacetylase/N-Sulfotransferase 1/NDST1 protein
Ser79-Arg882, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser79

Predicted Molecular Mass

93 kDa

SDS-PAGE

90-100 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to heparan sulfate.
The specific activity is >750 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5967-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Brij-35 and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: N-Deacetylase/N-Sulfotransferase 1/NDST1

Heparan sulfate is a highly sulfated polysaccharide that can be found on the cell surface and within extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans (1, 2). Heparin, on the other hand, is a highly sulfated version of heparan sulfate with no protein core and is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2-O position by heparan sulfate 2-O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3-O, and 6-O positions by N-deacetylase/N-sulfotransferases (NDSTs), heparan sulfate 3‑O sulfotransferases and heparan sulfate 6‑O sulfotransferases, respectively. All these enzymes are Golgi resident proteins. NDST-mediated modifications are believed to occur before modifications by other sulfotransferases (3). There are four NDSTs in the human genome and all are dual enzymes with both deacetylase and sulfotransferase domains (4, 5). NDST1 has both strong deacetylase and sulfotransferase activities. NDST1 deficiency causes developmental defects such as cerebral hypoplasia and craniofacial defects (6). The activity of this recombinant NDST1 is determined using a phosphatase coupled assay (7).

References

  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.

  2. Esko, J. D. and Selleck, S. B. (2002) Annu. Rev. Biochem. 71:435.

  3. Carlsson, P. et al. (2008) J. Biol. Chem. 283:20008.
  4. Aikawa, J. and Esko, J. D. (1999) J. Biol. Chem. 274:2690.

  5. Aikawa, J. et al. (2001) J. Biol. Chem. 276:5876.

      
  6. Grobe, K. et al. (2005) Development 132:3777.
  7. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Alternate Names

HSNST1, HSST1, NST1

Entrez Gene IDs

3340 (Human); 15531 (Mouse); 29633 (Rat)

Gene Symbol

NDST1

UniProt

P52848

Additional N-Deacetylase/N-Sulfotransferase 1/NDST1 Products

Product Documents for Recombinant Human NDST1 Protein, CF

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Product Specific Notices for Recombinant Human NDST1 Protein, CF

For research use only

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