Recombinant Human NDST2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7689-ST

R&D Systems, part of Bio-Techne
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7689-ST-010
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Key Product Details

Source

HEK293

Accession #

P52849

Conjugate

Unconjugated

Applications

Enzyme Activity

View all N-Deacetylase/N-Sulfotransferase 2/NDST2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human N-Deacetylase/N-Sulfotransferase 2/NDST2 protein
Ala81-Gly883, with C-terminal addition of HPGGGSGGGSGGGSHHHHHH

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala81

Predicted Molecular Mass

94 kDa

SDS-PAGE

90-100 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to heparan sulfate.
The specific activity is >1,250 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7689-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: N-Deacetylase/N-Sulfotransferase 2/NDST2

Heparan sulfate is a highly sulfated polysaccharide that can be found on the cell surface and within extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans (1, 2). Heparin, on the other hand, is a highly sulfated version of heparan sulfate and is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2-O position by heparan sulfate 2-O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3-O, and 6-O positions by N‑deacetylase/N-sulfotransferases (NDSTs), heparan sulfate 3‑O sulfotransferases and heparan sulfate 6‑O sulfotransferases, respectively. All these enzymes are Golgi resident proteins. NDST-mediated modifications are believed to occur before modifications by other sulfotransferases (3). There are four NDSTs in the human genome and all are dual enzymes with both deacetylase and sulfotransferase domains (4, 5). While NDST1 is mainly responsible for heparan sulfate synthesis, NDST2 is mainly responsible for the synthesis of heparin in mast cells (5). Disrupting the NDST2 gene causes production failure of fully sulfated heparin (6, 7).

References

  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
  2. Esko, J. D. and Selleck, S. B. (2002) Annu. Rev. Biochem. 71:435.
  3. Carlsson, P. et al. (2008) J. Biol. Chem. 283:20008.
  4. Aikawa, J. and Esko, J. D. (1999) J. Biol. Chem. 274:2690.
  5. Dagalv, A. et al. (2011) J. Biol. Chem. 286:44433.
  6. Forsberg, E. et al. (1999) Nature 400:773.
  7. Humphries, D.E. et al. (1999) Nature 400:769.

Alternate Names

NST2

Entrez Gene IDs

8509 (Human); 17423 (Mouse); 114002 (Rat)

Gene Symbol

NDST2

UniProt

P52849 (Human)

Additional N-Deacetylase/N-Sulfotransferase 2/NDST2 Products

Product Documents for Recombinant Human NDST2 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human NDST2 Protein, CF

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