Recombinant Human Nectin-1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10697-N1

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

Q15223.3

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Nectin-1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Nectin-1 protein
Human Nectin-1
(Gln31-Gly346)
Accession # Q15223.3		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln31, inferred from enzymatic deblocking revealing Val32

Predicted Molecular Mass

83 kDa

SDS-PAGE

78-88 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human Nectin-3  (Catalog # 3064-N3) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Nectin-1 Fc Chimera (Catalog # 10697-N1) binds with an ED50 of 2.5-20 ng/mL.

Scientific Data Images for Recombinant Human Nectin-1 Fc Chimera Protein, CF

Recombinant Human Nectin-1 Fc Chimera Protein Binding Activity.

When Recombinant Human Nectin-3 (3064-N3) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Nectin-1 Fc Chimera (Catalog # 10697-N1) binds with an ED50 of 2.5-20 ng/mL.

Recombinant Human Nectin-1 Fc Chimera Protein SDS PAGE.

2 μg/lane of Recombinant Human Nectin-1 Fc Chimera Protein (Catalog # 10697-N1) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-88 kDa and 156-176 kDa, respectively.

Formulation, Preparation and Storage

10697-N1
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-1

Nectin-1 (designated CD111), also called PRR-1 (poliovirus receptor-related protein 1) or HVEC (herpesvirus entry mediator C), is a widely expressed 110 kDa type I transmembrane glycoprotein that is important in the formation of adherens junctions and synapses. It is a member of the nectin family within the Ig superfamily (1, 2). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (2). The 517 amino acid (aa) human Nectin-1 isoform 1 contains a 30 aa signal sequence, a 325 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 141 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N-terminal V-type that mediates ligand binding, and two C2-type (3). Nectin-1, like other nectins, has a two splice forms: isoform 2 or HigR, 458 aa, with alternate TM and cytoplasmic sequences, and isoform 3, a 352 aa secreted protein (4). The common region of mature human Nectin-1 (aa 31-334) shares 93%, 94%, 96% and 96% aa identity with mouse, rat, bovine and porcine Nectin-1, respectively. Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4-7). In forming adherens junctions and synapses, Nectins 1 and 3 initiate cell-cell interactions, recruiting alpha v beta 3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8-11). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 down-regulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14). In colorectal cancer, Nectin-1 expression was found to be associated with a high risk for early disease recurrence (15).

References

  1. Lopez, M. et al. (1995) Gene 155:261.
  2. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
  3. Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
  4. Lopez, M. et al. (2001) J. Virol. 75:5684.
  5. Cocchi, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15700.
  6. Linehan, M. M. et al. (2004) J. Virol. 78:2530.
  7. Simpson, S. A. et al. (2005) J. Neurovirol. 11:208.
  8. Mizoguchi, A. et al. (2002) J. Cell Biol. 156:555.
  9. Togashi, H. et al. (2006) J. Cell Biol. 174:141.
  10. Tachibana, K. et al. (2000) J. Cell Biol. 150:1161.
  11. Takai, Y. and H. Nakanishi (2003) J. Cell Science 116:17.
  12. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
  13. Suzuki, K. et al. (2000) Nat. Genet. 25:427.
  14. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  15. Tampakis, A. et al. (2019) Oncology. 96:318.

Long Name

Poliovirus Receptor Related 1

Alternate Names

CD111, HVEC, Nectin1, PRR1, PVRL1, PVRR1

Entrez Gene IDs

5818 (Human); 58235 (Mouse); 102144697 (Cynomolgus Monkey)

Gene Symbol

NECTIN1

UniProt

Q15223.3

Additional Nectin-1 Products

Product Documents for Recombinant Human Nectin-1 Fc Chimera Protein, CF

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