Recombinant Human Nectin-1 His-tag Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI2880

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

Q15223.3

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all Nectin-1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Nectin-1 protein
Human Nectin-1
(Gln31-Gly346)
Accession # Q15223.3		 6-His tag Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln31 inferred from enzymatic pyroglutamate treatment revealing Val32. 

Predicted Molecular Mass

35 kDa

SDS-PAGE

50-65 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Nectin-3 Protein  (Catalog # 3064-N3) is immobilized at 3.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Nectin‑1 His-tag Avi-tag (Catalog # AVI2880) that produces 50% of the optimal binding response is 0.10-1.00 μg/mL.

Scientific Data Images for Recombinant Human Nectin-1 His-tag Avi-tag Protein, CF

Biotinylated Recombinant Human Nectin‑1 His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human Nectin‑1 His-tag Avi-tag Protein (Catalog # AVI2880) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-65 kDa.

Biotinylated Recombinant Human Nectin‑1 His-tag Avi-tag Protein Binding Activity.

When Recombinant Human Nectin-3 Protein (3064-N3) is immobilized at 3.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Nectin‑1 His-tag Avi-tag Protein (Catalog # AVI2880) that produces 50% of the optimal binding response is 0.100-1.00 μg/mL

Formulation, Preparation and Storage

AVI2880
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-1

Nectin-1, also called poliovirus receptor-related protein-1 (PRR1), is a member of the Nectin family which are Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. The Nectin family is comprised of 4 family members and 5 nectin-like molecules and they are structurally homologous to the poliovirus receptors (1). Mature human Nectin-1 consists of an extracellular domain (ECD) with three immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic domain that interacts with the F-actin–binding protein afadin (2). Within the ECD, human Nectin-1 shares 93% and 94% amino acid sequence identity with mouse and rat Nectin1, respectively. Two splice variants of Nectin-1 are known, one with an alternate cytoplasmic domain and a soluble form (4). Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4-7). In forming adherens junctions and synapses, Nectins-1 and -3 initiate cell-cell interactions, recruiting alpha v beta 3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8-11). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (1). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 down-regulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14). In colorectal cancer, Nectin-1 expression was found to be associated with a high risk for early disease recurrence (15). Our Avi-tag Biotinylated Nectin-1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
  2. Samanta, D. et al. (2012) PNAS 109:14836.
  3. Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
  4. Lopez, M. et al. (2001) J. Virol. 75:5684.
  5. Cocchi, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15700.
  6. Linehan, M. M. et al. (2004) J. Virol. 78:2530.
  7. Simpson, S. A. et al. (2005) J. Neurovirol. 11:208.
  8. Mizoguchi, A. et al. (2002) J. Cell Biol. 156:555.
  9. Togashi, H. et al. (2006) J. Cell Biol. 174:141.
  10. Tachibana, K. et al. (2000) J. Cell Biol. 150:1161.
  11. Takai, Y. and H. Nakanishi (2003) J. Cell Science 116:17.
  12. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
  13. Suzuki, K. et al. (2000) Nat. Genet. 25:427.
  14. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  15. Tampakis, A. et al. (2019) Oncology. 96:318.

Long Name

Poliovirus Receptor Related 1

Alternate Names

CD111, HVEC, Nectin1, PRR1, PVRL1, PVRR1

Entrez Gene IDs

5818 (Human); 58235 (Mouse); 102144697 (Cynomolgus Monkey)

Gene Symbol

NECTIN1

UniProt

Q15223.3

Additional Nectin-1 Products

Product Documents for Recombinant Human Nectin-1 His-tag Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human Nectin-1 His-tag Avi-tag Protein, CF

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