Recombinant Human Nectin-2/CD112 Fc Avi-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # AVI9317
Biotinylated
Key Product Details
Learn more about Avi-tag Biotinylated Proteins
Source
HEK293
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Biotin
Applications
Bioactivity
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human Nectin-2/CD112 protein
Human Nectin-2/CD112 (Gln32-Leu360) Accession # NP_002847.1 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
Avi-tag |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE with silver staining, under reducing conditions.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gln32 inferred from enzymatic pyroglutamate treatment revealing Asp33.
Predicted Molecular Mass
62 kDa
SDS-PAGE
74-84 kDa, under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human DNAM-1 Fc Chimera (Catalog # 666-DN) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Nectin-2/CD112 Fc Chimera Avi-tag binds with an ED50 of 8.00-48.0 ng/mL.
When Recombinant Human DNAM-1 Fc Chimera (Catalog # 666-DN) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Nectin-2/CD112 Fc Chimera Avi-tag binds with an ED50 of 8.00-48.0 ng/mL.
Scientific Data Images for Recombinant Human Nectin-2/CD112 Fc Avi-tag Protein, CF
Biotinylated Recombinant Human Nectin‑2/CD112 Fc Chimera Avi-tag Protein Binding Activity.
When Recombinant Human DNAM-1 Fc Chimera (666-DN) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Nectin-2/CD112 Fc Chimera Avi-tag (Catalog # AVI9317) binds with an ED50 of 8.00 - 48.0 ng/mL.Biotinylated Recombinant Human Nectin‑2/CD112 Fc Chimera Avi-tag Protein SDS-PAGE.
2 μg/lane of Biotinylated Recombinant Human Nectin‑2/CD112 Fc Chimera Avi-tag Protein (Catalog # AVI9317) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74 - 84 kDa and 148 - 168 kDa, respectively.Formulation, Preparation and Storage
AVI9317
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Nectin-2/CD112
References
- Eberle, F. et al. (1995) Gene 159:267.
- Samanta, D. and S.C. Almo (2015) Cell. Mol. Life Sci. 72:645.
- Samanta, D. et al. (2012) PNAS 109:14836.
- Struyf, F. et al. (2002) J. Virol. 76:12940.
- Bottino, C. et al. (2003) J. Exp. Med. 198:557.
- Pende, D. et al. (2005) Mol. Immunol. 42:463.
- Warner, M.S. et al. (1998) Virology 246:179.
- Satomi-Kobayashi, S. et al. (2009) Hypertension 54:825.
Long Name
Poliovirus Receptor Related 2
Alternate Names
CD112, HVEB, MPH, Nectin2, PRR2, PVRL2
Gene Symbol
NECTIN2
UniProt
Additional Nectin-2/CD112 Products
Product Documents for Recombinant Human Nectin-2/CD112 Fc Avi-tag Protein, CF
Product Specific Notices for Recombinant Human Nectin-2/CD112 Fc Avi-tag Protein, CF
For research use only
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