Recombinant Human Nectin-2/CD112 His-tag Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI2229

Biotinylated
R&D Systems, part of Bio-Techne
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AVI2229-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

NP_002847.1

Structure / Form


Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all Nectin-2/CD112 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Nectin-2/CD112 protein
Human Nectin-2
(Gln32-Leu360)
Accession # NP_002847.1		 6-His tag Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln32

Predicted Molecular Mass

38 kDa

SDS-PAGE

43-55 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human DNAM-1 Fc Chimera (Catalog # 666-DN) is immobilized at 2.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Nectin-2/CD112 His-tag Avi-tag protein binds with an ED50 of 0.600-3.60 µg/mL.

Scientific Data Images for Recombinant Human Nectin-2/CD112 His-tag Avi-tag Protein, CF

Biotinylated Recombinant Human Nectin‑2/CD112 His-tag Avi-tag Protein Binding Activity.

When Recombinant Human DNAM-1 Fc Chimera (666-DN) is immobilized at 2.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Nectin-2/CD112 His-tag Avi-tag Protein (Catalog # AVI2229) binds with an ED50 of 0.600-3.60 µg/mL.

Biotinylated Recombinant Human Nectin‑2/CD112 His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human Nectin‑2/CD112 His-tag Avi-tag Protein (Catalog # AVI2229) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-55 kDa.

Formulation, Preparation and Storage

AVI2229
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-2/CD112

Nectin-2, also known as Poliovirus receptor-related 2 (PRR2), is a member of the Nectin family which are Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions (1). The Nectin family is comprised of 4 family members and 5 nectin‑like molecules and they are structurally homologous to the poliovirus receptors (2). Mature human Nectin‑2 consists of an extracellular domain (ECD) with three immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic domain bind the F-actin–binding protein afadin (3). Within the ECD, human Nectin‑2 shares 72% amino acid (aa) sequence identity with mouse Nectin‑2. Alter native splicing generates an isoform with a truncated cytoplasmic tail (1). Nectin-2 localizes to adherens junctions between neurons, endothelial cells, epithelial cells, and fibroblasts (3, 4). It forms homodimers in cis, followed by dimers in trans (between cells) (4). It does not cis‑dimerize with other Nectins but forms cis‑dimers between its two splice forms. Notably, a Nectin-2 cis‑dimer on one cell can heterodimerize with a Nectin‑3 cis‑dimer on a neighboring cell (4). Nectin‑2 additionally binds to DNAM-1/CD226 on NK cells and triggers NK cell cytolytic activity (5, 6). Nectin‑2 is known to bind pseudorabies virus and herpes simplex virus-2 (HSV-2), but not HSV-1 or poliovirus (4, 7). Nectin‑2 is a component of cardiac intercalated discs and limits fibrosis and dysfunction resulting from pressure overload (8). Our Avi-tag Biotinylated Nectin‑2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Eberle, F. et al. (1995) Gene 159:267.
  2. Samanta, D. and S.C. Almo (2015) Cell. Mol. Life Sci. 72:645.
  3. Samanta, D. et al. (2012) PNAS 109:14836.
  4. Struyf, F. et al. (2002) J. Virol. 76:12940.
  5. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  6. Pende, D. et al. (2005) Mol. Immunol. 42:463.
  7. Warner, M.S. et al. (1998) Virology 246:179.
  8. Satomi-Kobayashi, S. et al. (2009) Hypertension 54:825.

Long Name

Poliovirus Receptor Related 2

Alternate Names

CD112, HVEB, MPH, Nectin2, PRR2, PVRL2

Entrez Gene IDs

5819 (Human); 19294 (Mouse); 102137456 (Cynomolgus Monkey)

Gene Symbol

NECTIN2

UniProt

NP_002847.1 (Human)

Additional Nectin-2/CD112 Products

Product Documents for Recombinant Human Nectin-2/CD112 His-tag Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Nectin-2/CD112 His-tag Avi-tag Protein, CF

For research use only

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