Recombinant Human Neudesin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6714-ND

R&D Systems, part of Bio-Techne
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6714-ND-050
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Key Product Details

Source

CHO

Accession #

Q9UMX5

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

View all Neudesin Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Neudesin protein
Gly32-Phe172, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Starts at Gly32

Predicted Molecular Mass

16.5 kDa

SDS-PAGE

19-21 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using Neuro-2A mouse neuroblastoma cells.
The ED50 for this effect is 1.5-6.0 μg/mL.

Formulation, Preparation and Storage

6714-ND
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neudesin

Neudesin (neuron‑derived neurotrophic secreted protein), gene name NENF (neudesin neurotrophic factor), also called CIR2 (cell immortalization‑related 2) or GIG47, is a secreted, 20‑21 kDa member of the MAPR (membrane‑associated progesterone receptor) subfamily of the cytochrome b5 family of molecules (1, 2). Human Neudesin is synthesized as a 172 amino acid (aa) precursor that contains a 31 aa signal sequence with a 141 aa mature region that possesses a cytochrome b5‑like heme‑binding domain over aa 44‑129, and a lysine acetylation site at aa 136 (1‑4). The attachment of hemin to its heme‑binding domain is necessary for its neurotrophic activity, and the binding of heme accounts for 5‑6 kDa of its circulating molecular weight (3). Mature human Neudesin shares 97%, 94% and 96% aa identity with mouse, rat, and bovine Neudesin, respectively. Neudesin appears to selectively promote cell survival or proliferation and inhibit differentiation in multiple settings. It is expressed by neuronal progenitors and neurons in the central nervous system, and by preadipocytes in white adipose tissue (5, 6). It promotes neuronal differentiation with limited proliferation and serves as a neuron survival factor, but inhibits both astrocyte and adipocyte differentiation (1, 5, 6). Neudesin over‑expression is found in tumors in the human breast, cervix, colon, lung and skin, and in human tumor cell lines that include some lymphomas and leukemias; transfection studies indicate that it may act as an oncogene (4, 7).

References

  1. Kimura, I. et al. (2005) J. Neurosci. Res. 79:287.
  2. Ma, L. et al. (1998) Oncogene 17:1321.
  3. Kimura, I. et al. (2008) J. Biol. Chem. 283:4323.
  4. Han, K.H. et al. (2012) BMC Cancer 12:274.
  5. Kimura, I. et al. (2006) J. Neurosci. Res. 83:1415.
  6. Kimura, I. et al. (2009) Biochem. Biophys. Res. Commun. 381:75.
  7. Neubauer, H. et al. (2006) Electrophoresis 27:1840.

Alternate Names

CIR2, NENF, SCIRP10, SPUF

Entrez Gene IDs

29937 (Human); 66208 (Mouse); 289380 (Rat)

Gene Symbol

NENF

UniProt

Q9UMX5 (Human)

Additional Neudesin Products

Product Documents for Recombinant Human Neudesin Protein, CF

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Product Specific Notices for Recombinant Human Neudesin Protein, CF

For research use only

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