Recombinant Human NFATC2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9716-NA

R&D Systems, part of Bio-Techne
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9716-NA-050
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Key Product Details

Source

E. coli

Accession #

Q13469-1

Conjugate

Unconjugated

View all NFATC2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human NFATC2 protein
His575-Pro679, with an N-terminal Met and a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

13 kDa

SDS-PAGE

16 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9716-NA
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution
Reconstitute at 500 μg/mL in 4 mM HCl..

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFATC2

Nuclear factor of activated T cells, cystoplamic 2 (NFATC2), also known as T cell transcription factor NFAT1, is a member of the calcium-regulated family of transcription factors that regulate gene expression in lymphocytes and control cardiac valve formation (1, 2). The NFAT family is comprised of four members, NFATC1-C4. Human NFATC2 is a 925 aa transcription factor containing several key regions including a calcineurin-binding site, a trans-activation domain, and nuclear localization and export signals. The first three members of this family are thought to be immune-specific forms of NFAT, while NFATC4 has functions in the regulation of cardiac hypertrophy and hippocampal neuronal signaling (3, 4). In resting T cells, the NFAT proteins are present in the cytoplasm in their phosphorylated form. Upon elevation of intracellular calcium concentration and activation of the phosphatase calcineurin, the NFATC proteins are dephosphorylated and translocated into the nucleus where they regulate the transcription of genes for a number of cytokines and surface molecules (5, 6).

References

  1. Rengarajan, J. et al. (2002) Nat. Immunol. 3:48.
  2. Baksh, S. et al. (2002) Mol. Cell. 10:1071.
  3. Molkentin, J.D. et al. (1998) Cell. 93:215.
  4. Graef, I.A. et al. (1999) Nature. 401:703.
  5. Bopp, T. et al. (2005) J. Exp. Med. 201:181.
  6. Ranger, A.M. et al. (2000) J. Exp. Med. 191:9.

Long Name

Nuclear factor of activated T-cells, cytoplasmic 2

Alternate Names

NFAT1, NFATP

Entrez Gene IDs

4773 (Human)

Gene Symbol

NFATC2

UniProt

Q13469-1

Additional NFATC2 Products

Product Documents for Recombinant Human NFATC2 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human NFATC2 Protein, CF

For research use only

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