Recombinant Human NG2/MCSP/CSPG4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2585-PG

R&D Systems, part of Bio-Techne
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2585-PG-050
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Key Product Details

Source

NS0

Accession #

Q6UVK1

Conjugate

Unconjugated

Applications

Bioactivity

View all NG2/MCSP Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human NG2/MCSP protein
Ser1583-Ser2224, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser1583

Predicted Molecular Mass

69 kDa

SDS-PAGE

100-110 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 μL droplet containing 90 ng on a nitrocellulose coated microplate.

Formulation, Preparation and Storage

2585-PG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 300 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NG2/MCSP

NG2, also known as CSPG4, MCSP, and AN2, is a 400-500 kDa transmembrane chondroitin sulfate proteoglycan (CSPG) with a protein core of approximately 300 kDa. The extracellular region can be proteolytically shed from the cell surface (1-3). Mature human NG2 consists of a 2195 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain (4). Within aa 1583-2224, human NG2/CSPG4 shares 83% aa sequence identity with mouse and rat CSPG4. NG2 binds to the extracellular matrix proteins Laminin, Tenascin, and Collagens II, V, and VI as well as to the growth factors FGF-2 and PDGF-AA (1, 5, 6). NG2 is expressed on glial cell progenitors known as O2A cells or NG2 glia (2, 7, 8). These cells are neuronally responsive and differentiate primarily into oligodendrocytes but also into astrocytes (8-10). NG2 associates with PDGF R alpha and the AMPA R subunit GluR2 (2, 7). It is up-regulated on microglial cells during inflammation and contributes to the induction of inflammatory mediators (11). Various CSPGs in the brain inhibit neurite outgrowth through interactions with Nogo Receptor/NgR1 and NgR3 (12). This recombinant protein product corresponds to the last 5 CSPG repeats, a region which can independently inhibit neurite outgrowth (13). NG2 is also expressed on vascular mural cells and capillaries (10, 14). It promotes vascular endothelial cell (EC) migration and angiogenesis through interactions with Galectin-3 and Integrin alpha3 beta1 on EC, Plasminogen, and Angiostatin (15, 16). NG2 is also expressed on a variety of tumors where it contributes to tumor cell adhesion, motility, and invasion (17).

References

  1. Campoli, M. et al. (2010) Adv. Cancer Res. 109:73.
  2. Nishiyama, A. et al. (1996) J. Neurosci. Res. 43:315.
  3. Nishiyama, A. et al. (1995) Mol. Biol. Cell 6:1819.
  4. Pluschke, G. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9710.
  5. Burg, M.A. et al. (1996) J. Biol. Chem. 271:26110
  6. Goretzki, L. et al. (1999) J. Biol. Chem. 274:16831.
  7. Stegmuller, J. et al. (2003) J. Biol. Chem. 278:3590.
  8. Komitova, M. et al. (2009) J. Comp. Neurol. 512:702.
  9. Bergles, D.E. et al. (2000) Nature 405:187.
  10. Zhu, X. et al. (2008) Development 135:145.
  11. Gao, Q. et al. (2010) Neuroscience 165:386.
  12. Dickendesher, T.L. et al. (2012) Nat. Neurosci. 15:703.
  13. Ughrin, Y.M. et al. (2003) J. Neurosci. 23:175.
  14. Schlingemann, R.O. et al. (1990) Am. J. Pathol. 136:1393.
  15. Fukushi, J. et al. (2004) Mol. Biol. Cell 15:3580.
  16. Goretzki, L. et al. (2000) J. Biol. Chem. 275:28625.
  17. Wang, X. et al. (2010) Curr. Mol. Med. 10:419.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Alternate Names

AN2, CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG

Entrez Gene IDs

1464 (Human); 121021 (Mouse); 81651 (Rat)

Gene Symbol

CSPG4

UniProt

Q6UVK1

Additional NG2/MCSP Products

Product Documents for Recombinant Human NG2/MCSP/CSPG4 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human NG2/MCSP/CSPG4 Protein, CF

For research use only

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