Recombinant Human Notch-4 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9909-NT

R&D Systems, part of Bio-Techne
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Key Product Details

Source

T. ni (baculovirus)

Accession #

Q99466-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Notch-4 Proteins and Enzymes »

Product Specifications

Source

Trichoplusia ni, T. ni (baculovirus)-derived human Notch-4 protein
Human Notch‑4
(Arg24-Glu549)
Accession # Q99466-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg24

Predicted Molecular Mass

82 kDa

SDS-PAGE

94-116 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human DLL4 (Catalog # 1506-D4) is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Notch-4 Human Fc Chimera that produces 50% of the optimal binding response is 0.5-2.5 μg/mL.

Scientific Data Images for Recombinant Human Notch-4 Fc Chimera Protein, CF

Recombinant Human Notch-4 Fc Chimera Protein Binding Activity

Recombinant Human Notch-4 Fc Chimera Protein Binding Activity

When Recombinant Human DLL4 (Catalog # 1506-D4) is immobilized at 2 ug/mL, Recombinant Human Notch-4 Fc Chimera (Catalog # 9909-NT) binds with an ED50 of 0.5-2.5 µg/mL.
Recombinant Human Notch-4 Fc Chimera Protein SDS-PAGE

Recombinant Human Notch-4 Fc Chimera Protein SDS-PAGE

2μg/lane of Recombinant Human Notch‑4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 94-116 kDa and 170-200 kDa, respectively.

Formulation, Preparation and Storage

9909-NT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Notch-4

Human Notch-4 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes (1-3). Human Notch-4 is synthesized as a 2003 amino acid (aa) precursor that contains a 23 aa signal sequence, a 1424 aa extracellular domain (ECD) with 29 EGF-like repeats and three Lin-12/Notch repeats (LNR), a 21 aa transmembrane (TM) segment and a 535 aa cytoplasmic domain containing five ankyrin repeats (1, 3). Within the expressed region of this product (including EGF domains 1-13) human Notch-4 shares 84%, 83%, 51%, and 48% aa sequence identity with mouse Notch-4, rat Notch-4, human Notch-2 and human Notch-1 respectively. The 11th and 12th EGF-like repeats bind ligands including Jagged and Delta-like families in humans (4). Delta-like ligand-4 (DLL4)-Notch-4 signaling correlates to angiogenesis in glioblastomas (5). Notch-4 has been identified as a key player in invasive cancers such as breast cancer, hepatocellular carcinoma, melanoma, and prostate cancer (6-9). Notch-4 expression in 3T3L preadipocytes enhanced their proliferation and adipogenesis though up‑regulation of adipogenic genes such as C/EBP alpha, PPAR gamma, aP2, LPL and HSL (10). It has also been shown to be involved in proliferation of gastric cancer though Wnt-1/ beta-catenin signaling regulation (11).

References

  1. Dumortier, A. et al. (2005) Int. J. Hematol. 82:277.
  2. Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709.
  3. Leong, K.G. and A. Karsan (2006) Blood 107:2223.
  4. Hambleton, S. et al. (2004) Structure 12:2173.
  5. Zhang, J.F. et al. (2016) Tumour Biol. 37:3797.
  6. Yao, K. et al. (2011) Int. J. Surg. Pathol. 19:607.
  7. Gramantieri, L. et al. (2007) Liver Int. 27:997.
  8. Lin, X. et al. (2016) Cancer Sci. 107:1079.
  9. Zhang, J. et al. (2017) Apoptosis. 22:877.
  10. Lai, P.Y. et al. (2013) Biochem. Biophys. Res. Commun. 430:1132.
  11. Qian, C. et al. (2015) Mol. Cell. Biochem. 401:165.

Alternate Names

INT3, Notch4

Entrez Gene IDs

4855 (Human); 18132 (Mouse); 406162 (Rat)

Gene Symbol

NOTCH4

UniProt

Q99466-1

Additional Notch-4 Products

Product Documents for Recombinant Human Notch-4 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Notch-4 Fc Chimera Protein, CF

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