Recombinant Human NPEPPS Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6410-ZN

R&D Systems, part of Bio-Techne
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6410-ZN-010
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P55786

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Puromycin-sensitive aminopeptidase/NPEPPS Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Puromycin-sensitive aminopeptidase/NPEPPS protein
Pro46-Val919, with an N-terminal Met and a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro46

Predicted Molecular Mass

100 kDa

SDS-PAGE

90-100 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Leu-AMC.
The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6410-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Puromycin-sensitive aminopeptidase/NPEPPS

NPEPPS, also known as Puromycin-sensitive aminopeptidase and cytosol alanyl aminopeptidase, is a zinc metallopeptidase which hydrolyzes N‑terminal amino acids from its substrates. NPEPPS is expressed in most tissues as a cytoplasmic protein, but a membrane-associated form has been identified in the brain (1, 2). The enzyme has broad substrate specificity but prefers basic and hydrophobic residues in the substrate P1 and P'1 sites (3). NPEPPS is known to hydrolyze dynorphins and enkephalins, and may contribute to the degradation of these peptides in the brain (4). NPEPPS plays a major role in the degradation of toxic polyglutaminyl peptides that are released by proteasomes (5). NPEPPS is also known to degrade the tau protein, which accumulates and polymerizes in some neurodegenerative diseases (6).

References

  1. Dyer, S.H. et al. (1990) J. Neurochem. 54:547.
  2. McLellan, S. et al. (1988) J. Neurochem. 51:1552.
  3. Johnson, G.D. and L.B. Hersh (1990) Arch. Biochem. Biophys. 276:305.
  4. Safavi, A. and L.B. Hersh (1995) J. Neurochem. 65:389.
  5. Bhutani, N. et al. (2007) EMBO J. 26:1385.
  6. Sengupta, S. et al. (2006) Biochemistry 45:15111.

Alternate Names

AAP-S, MP100, NPEPPS

Entrez Gene IDs

9520 (Human)

Gene Symbol

NPEPPS

UniProt

P55786

Additional Puromycin-sensitive aminopeptidase/NPEPPS Products

Product Documents for Recombinant Human NPEPPS Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human NPEPPS Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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