Recombinant Human NQO-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7567-DH

R&D Systems, part of Bio-Techne
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7567-DH-010
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Key Product Details

Source

E. coli

Accession #

P15559

Conjugate

Unconjugated

Applications

Enzyme Activity

View all NQO-1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human NQO-1 protein
Met1-Lys274, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

32 kDa

SDS-PAGE

29-31 kDa, reducing conditions

Activity

Measured by its ability to oxidize Resazurin.
The specific activity is >15,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7567-DH
Formulation Supplied as a 0.2 μm filtered solution in NaH2PO4 and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: NQO-1

NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1), also known as DT-diaphorase, is a widely-distributed FAD-dependent flavoprotein that promotes 2-electron reductions of quinones, quinoneimines, nitroaromatics, and azo dyes (1, 2). As a result it prevents the one electron reduction of quinones that results in the production of radical species. NQO1 is a highly-inducible enzyme that is regulated by the Keap1/Nrf2/ARE pathway (2, 3). The increase and decrease of NQO1 levels are associated with decreased and increased susceptibilities to oxidative stress, respectively. Thus, NQO1 is a marker cytoprotective enzyme in oxidative stress. Independently of its catalytic function, NQO1 plays a role in regulating the proteosomal degradation of p53, p73a, and p33 (2). NQO1 physically interacts with p53 and p73 in an NADH-dependent manner and protects them from 20S proteasomal degradation in a ubiquitin independent pathway (4).

References

  1. Gong, X. et al. (2008) Vitamin Horm. 78:85.
  2. Dinkova-Kostova, A. T., and Talalay, P. (2010) Arch. Biochem. Biophys. 501:116.
  3. Hayes, J. D. and McMahon, M. (2009) Trends Biochem. Sci. 34:176.
  4. Asher, G. et al. (2005) Genes Dev. 19:316.

Long Name

NAD(P)H Dehydrogenase, Quinone 1

Alternate Names

Azoreductase, DHQU, DIA4, Diaphorase-4, Dioxin-inducible 1, DTD, Menadione Reductase, NMOR1, NQO1, QR1, Quinone Reductase 1

Entrez Gene IDs

1728 (Human)

Gene Symbol

NQO1

UniProt

P15559 (Human)

Additional NQO-1 Products

Product Documents for Recombinant Human NQO-1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human NQO-1 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd. Tween is a registered trademark of ICI Americas.

For research use only

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