Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain Protein Best Seller
R&D Systems, part of Bio-Techne | Catalog # 396-HB
Key Product Details
Product Specifications
Source
Thr176-Lys246
Purity
Endotoxin Level
N-terminal Sequence Analysis
Thr176
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 0.06-0.3 ng/mL.
Scientific Data Images for Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain Protein
Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain Protein Bioactivity
Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain (Catalog # 396-HB) stimulates cell proliferation of the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.06-0.3 ng/mL.Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain Protein SDS-PAGE
1 μg/lane of Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 396-HB
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 396-HB/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Neuregulin-1 beta 1/NRG1 beta 1
The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 has been shown to encode more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. All NRG1 isoforms contain an EGF-like domain ( alpha- or beta-splice variant that differ in their C-terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF) NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1s (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.
References
- Buonanno, A., and Fischbach, G.D. (2001) Curr. Opin. Neurobiol. 11:287.
- Adlkofer, K. and Lai, C. (2000) Glia 29:104.
- Garratt, A.N. et al. (2000) BioEssays 22:987.
Alternate Names
Gene Symbol
UniProt
Additional Neuregulin-1 beta 1/NRG1 beta 1 Products
Product Documents for Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain Protein
Product Specific Notices for Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain Protein
For research use only