Recombinant Human NRG1 isoform 9/GGF-2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11505-NR

R&D Systems, part of Bio-Techne
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11505-NR-050
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Key Product Details

Source

CHO

Accession #

Q02297-9

Conjugate

Unconjugated

Applications

Bioactivity

View all Neuregulin-1/NRG1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Neuregulin-1/NRG1 protein
(isoform 9/GGF-2) Gly51-Glu422
Accession # Q02297-9

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly51

Predicted Molecular Mass

40 kDa

SDS-PAGE

70-80 kDa, under reducing conditions.

Activity

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.
The ED50 for this effect is 0.750-7.50 ng/mL.

Scientific Data Images for Recombinant Human NRG1 isoform 9/GGF-2 Protein, CF

Recombinant Human NRG1 isoform 9/GGF-2 Protein Bioactivity.

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 0.750-7.50 ng/mL.

Recombinant Human NRG1 isoform 9/GGF-2 Protein SDS-PAGE.

2 μg/lane of Recombinant Human NRG1 isoform 9/GGF-2 Protein (Catalog # 11505-NR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-80 kDa, under reducing conditions.

Formulation, Preparation and Storage

11505-NR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuregulin-1/NRG1

Glial growth factor2, GGF-2, also known as cimaglermin alfa or NRG1 beta3 (1), is a full-length splice variant of the Neuregulin-1 (NRG1) family gene. NRG1 is one of the four members  of the neuregulin family of genes (NRG1-4) encoding signaling proteins that facilitate cell–cell interactions in many tissues, including the nervous system and the heart (2). All family members share an EGF-like domain that interacts with the ErbB family of tyrosine kinase receptors. Through their displayed interaction with ERBB receptors, NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells. The ErbB2 and ErbB4 receptors cooperate in transmission of neuregulin-1 signals in the heart, whereas ErbB2 and ErbB3 cooperate in neural crest cells.Neuregulins are emerging as potential treatments for heart failure. GGF-2 was chosen as a development candidate with potentially long-lasting pharmacodynamic activity (4).

References

  1. Dimayuga, F.O. et al. (2003) J Neuroimmunol 136:67.
  2. Falls D.L. (2003). Exp. Cell Res. 284:14.
  3. Marchionni M.A. et al. (1999) Adv Exp Med Biol. 468:283.
  4. Caggiano A.O. et al. (2017) Eur J Pharmacol.796:76.

Alternate Names

Heregulin-1, HGL, HRG1-alpha, HRG1-beta 1, Neuregulin1, NRG1

Entrez Gene IDs

3084 (Human); 211323 (Mouse); 112400 (Rat)

Gene Symbol

NRG1

UniProt

Q02297-9

Additional Neuregulin-1/NRG1 Products

Product Documents for Recombinant Human NRG1 isoform 9/GGF-2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human NRG1 isoform 9/GGF-2 Protein, CF

For research use only

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