Recombinant Human OX40/TNFRSF4 Fc Alexa Fluor® 488 Protein

R&D Systems, part of Bio-Techne | Catalog # AFG3388

R&D Systems, part of Bio-Techne
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AFG3388-020
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Key Product Details

Learn more about FluorokinesTM Fluorescent-Labeled Proteins

Source

NS0

Accession #

P43489.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Bioactivity

View all OX40 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human OX40/TNFRSF4 protein
Human OX40
(Leu29-Ala216)
Accession # P43489.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu29

Predicted Molecular Mass

46.7 kDa (monomer)
Labeled with Alexa Fluor® 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515­-545 nm

SDS-PAGE

70 kDa, under reducing conditions.

Activity

Measured by flow cytometry for its ability to bind anti-Human OX40/TNFRSF4 Monoclonal Antibody conjugated beads.The concentration of Recombinant Human OX40/TNFRSF4 Fc Chimera Alexa Fluor® 488 (Catalog # AFG3388) that produces 50% of the binding response is 15.0-150 ng/mL

Scientific Data Images for Recombinant Human OX40/TNFRSF4 Fc Alexa Fluor® 488 Protein

Flow cytometry analysis for Recombinant Human OX40/TNFRSF4 Fc Chimera Alexa Fluor® 488 staining on anti-Human OX40/TNFRSF4 Monoclonal Antibody conjugated beads.

Streptavidin coated beads conjugated to biotinylated anti-Human OX40/TNFRSF4 Monoclonal Antibody were stained with the indicated concentrations of Recombinant Human OX40/TNFRSF4 Fc Chimera Alexa Fluor® 488 (Catalog# AFG3388).

Recombinant Human OX40/TNFRSF4 Fc Chimera Alexa Fluor® 488 Protein SDS-PAGE.

2 μg/lane of Recombinant Human OX40/TNFRSF4 Fc Chimera Alexa Fluor® 488 Protein (Catalog # AFG3388) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70 kDa and 140 kDa, respectively.

Formulation, Preparation and Storage

AFG3388
Formulation Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: OX40

OX40 (CD134; TNFRSF4) is a T cell co-stimulatory molecule of the TNF receptor superfamily that coordinates with other co-stimulators (CD28, CD40, CD30, CD27 and 4-1BB) to manage the activation of the immune response (1-3). Human OX40 is a 48 kDa type I transmembrane glycoprotein with a 28 amino acid (aa) signal sequence, a 185 aa extracellular domain (ECD) that contains a cysteine-rich region, a 20 aa transmembrane segment, and a 41 aa cytoplasmic domain (4). The ECD of human OX40 shares 63% sequence identity with the ECD of mouse and rat OX40. OX40 is up-regulated on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7 (5, 6). OX40 Ligand is primarily expressed on antigen presenting cells (5). OX40 Ligand engagement of OX40 on activated CD4+ T cells results in increased T cell survival, proliferation, and cytokine production. It also inhibits the conversion of effector T cells into immunosuppressive regulatory T cells (Tregs) and can promote the maintenance of and recall response in memory T cells (3, 7-10). OX40 is constitutively expressed on Tregs and enhances the sensitivity of Tregs to IL-2, thus promoting Treg proliferation. OX40 has also been shown to decrease the cells’ immunosuppressive activity on effector T cells (11-14). OX40-OX40 Ligand signaling is involved in allergic airway inflammation, graft-versus-host disease and autoimmune disease (6, 15, 16). Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease (17, 18).

References

  1. Hori, T. (2006) Int. J. Hematol. 83:17.
  2. Latza, U. et al. (1994) Eur. J. Immunol. 24:677.
  3. Salek-Ardakani, S. et al. (2003) J. Exp. Med. 198:315.
  4. al-Shamkhani, A. et al. (1996) Eur. J. Immunol. 26:1695.
  5. Moran, A.E. et al. (2013) Curr. Opin. Immunol. 25:230.
  6. Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
  7. Xiao, X. et al. (2008) J. Immunol. 181:3193.
  8. So, T. and M. Croft (2007) J. Immunol. 179:1427.
  9. Mousavi, S.F. et al. (2008) J. Immunol. 181:5990.
  10. Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907.
  11. Piconese, S. et al. (2010) Eur. J. Immunol. 40:2902.
  12. Griseri, T. et al. (2010) J. Exp. Med. 207:699.
  13. Xiao, X. et al. (2012) J. Immunol. 188:892.
  14. Vu, M.D. et al. (2007) Blood 110:2501.
  15. Damayanti, T. et al. (2010) Am. J. Respir. Crit. Care Med. 181:688.
  16. Xiao, X. et al. (2012) Nat. Immunol. 13:981.
  17. Nakano, M. et al. (2010) Cardiovasc. Res. 88:539.
  18. Ishii, N. et al. (2010) Adv. Immunol. 105:63.
  19. Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757

Alternate Names

ACT-135, CD134, Ly-70, TNFRSF4, Txgp1, TXGP1L

Entrez Gene IDs

7293 (Human); 22163 (Mouse); 25572 (Rat); 102145978 (Cynomolgus Monkey)

Gene Symbol

TNFRSF4

UniProt

P43489.1

Additional OX40 Products

Product Documents for Recombinant Human OX40/TNFRSF4 Fc Alexa Fluor® 488 Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human OX40/TNFRSF4 Fc Alexa Fluor® 488 Protein

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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