Recombinant Human PA28 Activator alpha Subunit Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E-381

R&D Systems, part of Bio-Techne
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E-381-100
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Key Product Details

Source

E. coli

Accession #

Q06323.1

Conjugate

Unconjugated

Applications

Bioactivity

View all PA28 Activator alpha Subunit Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human PA28 Activator alpha Subunit protein
Met1 - Tyr249

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

29 kDa

Activity

Recombinant Human PA28 Activator alpha Subunit is ideal for the activation of latent 20S Proteasome complexes. Reaction conditions will need to be optimized for each specific application. Initially, we recommend a 5- to 15-fold molar excess of Recombinant Human PA28 Activator alpha Subunit relative to 20S Proteasome.

Formulation, Preparation and Storage

E-381
Formulation Supplied as a solution in HEPES, NaCl, DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: PA28 Activator alpha Subunit

The Proteasome Activator 28 (PA28) Regulatory Complex, also known as the 11S Regulatory (REG) Complex, is a ring-shaped, multimeric, ATP-independent regulatory complex that can bind to one or both ends of the 20S Proteasome or 20S Immunoproteasome (1,2). The PA28 Regulatory Complex stimulates the proteasome to hydrolyze small peptides, but not ubiquitinated proteins, for the production of peptides for MHC class I antigen presentation (3-7). Three PA28 Regulatory Complex subunits, alpha, beta, and gamma, have been identified and can oligomerize to form the PA28 Regulatory Complex (8-10). While the expression of the PA28 alpha and beta subunits are induced by IFN-gamma, the PA28 gamma subunit is not significantly affected (11,12).  In vivo, PA28 alpha forms hetero-heptamers or -hexamers with PA28 beta (13-15). PA28 alpha can also form active homo-heptamers in vitro and can be used to activate the 20S Proteasome (3,16). In addition to regulating production of MHC class I antigens, PA28 alpha may protect against oxidative stress (17).

References

  1. Cascio, P. et al. (2002) EMBO J. 21:2636.
  2. Shibatani, T. et al. (2006) Mol. Biol. Cell 17:4962.
  3. Ma, C.P. et al. (1992) J. Biol. Chem. 267:10515.
  4. Wilk, S. et al. (2000) Arch. Biochem. Biophys. 383:265.
  5. Knowlton, J.R. et al. (1997) Nature 390:639.
  6. Groettrup, M. et al. (1996) Nature 381:166.
  7. Dick, T.P. et al. (1996) Cell 86:253.
  8. Kuehn, L. & B. Dahlmann (1997) Mol. Biol. Rep. 24:89.
  9. Li, J. & M. Rechsteiner (2001) Biochimie 83:373.
  10. Realini, C. et al. (1997) J. Biol. Chem. 272:25483.
  11. Jiang, H. & J.J. Monaco (1997) Immunogenetics 46:93.
  12. Ahn, J.Y. et al. (1995) FEBS Lett. 366:37.
  13. Zhang, Z. et al. (1999) Biochemistry 38:5651.
  14. Song, X. et al. (1996) J. Biol. Chem. 271:26410.
  15. Mott, J.D. et al. (1994) J. Biol. Chem. 269:31466.
  16. Johnston, S.C. et al. (1997) Protein Sci. 6:2469.
  17. Li, J. et al. (2011) FASEB J. 25:883.

Long Name

Proteasome (Prosome, Macropain) Activator Subunit 1

Alternate Names

11S Regulator Complex Subunit alpha, IFI5111, PSME1, REG alpha

Entrez Gene IDs

5720 (Human); 19186 (Mouse); 29630 (Rat)

Gene Symbol

PSME1

UniProt

Q06323.1

Additional PA28 Activator alpha Subunit Products

Product Documents for Recombinant Human PA28 Activator alpha Subunit Protein, CF

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Product Specific Notices for Recombinant Human PA28 Activator alpha Subunit Protein, CF

For research use only

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