Recombinant Human PANK1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11161-PK

R&D Systems, part of Bio-Techne
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11161-PK-020
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Key Product Details

Source

E. coli

Accession #

Q8TE04.2

Conjugate

Unconjugated

Applications

Enzyme Activity

View all PANK1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human PANK1 protein
Lys231-Lys598, with a N-terminal Met & 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

42 kDa

SDS-PAGE

40 kDa, under reducing conditions.

Activity

Measured by its ability to transfer phosphate from ATP to pantothenic acid.
The specific activity is >170 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human PANK1 His-tag Protein, CF

Recombinant Human PANK1 His-tag Protein Enzyme Activity Diagram

Recombinant Human PANK1 His-tag Protein SDS-PAGE

1 μg/lane of rhPANK1 (Catalog # 11161-PK) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 40 kDa.

Formulation, Preparation and Storage

11161-PK
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: PANK1

The pantothenate kinase (PANK) gene family contains four members and encodes five functional isoforms. The PANK enzymes are key to the synthesis of coenzyme A (CoA) (1,2). CoA is used by around 4% of intracellular enzymes as a co-substrate. PANK1 is the key regulatory enzyme in the CoA biosynthetic pathway and plays similar roles in both bacterial and mammalian cells (3). PANK1 expression levels are high in liver, kidney, and heart tissues (4). Mutations in PANK1 have been linked to Hallervorden-Spatz syndrome, an autosomal recessive neurodegenerative disorder caused by excessive brain iron accumulation due to the defects in CoA synthesis (5). The activity of this enzyme has been measured with a phosphatase-coupled method (6).

References

  1. Zhou, B. et al. (2001) Nat Genet. 28:345. 
  2. Polsterm, B.J. et al. (2010) Gene  465:53. 
  3. Robishaw, J.D. et al. (1985) Am. J. Physiol. 1:248.
  4. Rock, C.O. et al. (2000). J Biol Chem. 275:1377.
  5. Gordon, N. (2002) Eur J Paediatr Neurol. 6:243. 
  6. Wu, Z.L. (2011) PLoS ONE 6:e23172.

Long Name

Pantothenate Kinase 1

Alternate Names

HPanK, HPanK1, MGC24596, PANK, PANK1a, PANK1b

Entrez Gene IDs

53354 (Human); 75735 (Mouse)

Gene Symbol

PANK1

UniProt

Q8TE04.2

Additional PANK1 Products

Product Documents for Recombinant Human PANK1 His-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human PANK1 His-tag Protein, CF

For research use only

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