Recombinant Human PANP Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7920-PN

R&D Systems, part of Bio-Techne
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7920-PN-050
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Key Product Details

Source

HEK293

Accession #

Q8IYJ0

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all PANP Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human PANP protein
Human PANP
(Ser76-Pro178)
Accession # Q8IYJ0		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser76 & Val80

Predicted Molecular Mass

37 kDa (monomer)

SDS-PAGE

58-69 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.
The ED50 for this effect is 25-100 ng/mL.

Formulation, Preparation and Storage

7920-PN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PANP

PILR‑alpha associated neural protein (PANP) is an approximately 30 kDa type 1 transmembrane glycoprotein that contains a 147 amino acid (aa) extracellular domain (ECD) and an 83 aa cytoplasmic domain (1). Within the ECD, human PANP shares 93% aa sequence identity with mouse and rat PANP. Alternative splicing may generate an additional isoform with a 6 aa truncation at the C‑terminus. PANP is preferentially expressed in the central nervous system (1). It binds to PILR‑alpha, an inhibitory receptor that is expressed on dendritic cells, neutrophils, and macrophages (1, 2). PILR‑alpha additionally binds to CD99, NPDC‑1, CL‑P1/COLEC12, and glycoprotein B of Herpes simplex virus type 1 (3‑5). PILR‑alpha binding requires the presence of sialylated O‑linked carbohydrates on each of these ligands, including PANP (1, 3, 4, 6). The in cis association of PILR‑alpha with sialylated molecules on the neutrophil surface inhibits beta integrin activation and neutrophil extravasation during inflammatory responses (7).

References

  1. Kogure, A. et al. (2011) Biochem. Biophys. Res. Commun. 405:428.
  2. Fournier, N. et al. (2000) J. Immunol. 165:1197.
  3. Wang, J. et al. (2008) J. Immunol. 180:1686.
  4. Sun, Y. et al. (2012) J. Biol. Chem. 287:15837.
  5. Satoh, T. et al. (2008) Cell 132:935.
  6. Wang, J. et al. (2009) J. Virol. 83:13042.
  7. Wang, J. et al. (2013) Nat. Immunol. 14:34.

Long Name

PILR alpha Associated Neural Protein

Alternate Names

C12orf53, PIANP

Entrez Gene IDs

196500 (Human); 319352 (Mouse); 312711 (Rat)

Gene Symbol

PIANP

UniProt

Q8IYJ0

Additional PANP Products

Product Documents for Recombinant Human PANP Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human PANP Fc Chimera Protein, CF

For research use only

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