Recombinant Human PCPE-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2627-PE

R&D Systems, part of Bio-Techne
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2627-PE-020
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Key Product Details

Source

NS0

Accession #

Q15113

Conjugate

Unconjugated

Applications

Enzyme Activity

View all PCPE-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human PCPE-1 protein
Met1-Asp449, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No result, Gln26 expected

Predicted Molecular Mass

47 kDa

SDS-PAGE

51-57 kDa, reducing conditions

Activity

Measured by the stimulation of procollagen type I processing by BMP-1.
>50% of the full-length procollagen, in the presence of Recombinant Human PCPE-1, is processed by BMP-1, as measured under the described conditions.

Formulation, Preparation and Storage

2627-PE
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: PCPE-1

Procollagen C‑endopeptidase enhancers, known as PCPEs or PCOLCEs, are secreted extracellular matrix glycoproteins that consist of two CUB domains and one NTR domain. They are known to stimulate enzymatic cleavage of procollagens I‑III by the BMP‑1/tolloid family of metalloproteases, also known as procollagen C‑proteinases (1). PCPE‑1 is expressed primarily by interstitial connective tissues such as tendons, calvaria, and skin (2). Although BMP‑1/tolloid proteinases are involved in processing of multiple extracellular proteins, the enhancer activity of PCPE‑1 is specific to procollagens since it has no effect on BMP‑1/tolloid cleavage of other substrates (3). It is thought that PCPE‑1 enhances cleavage of procollagens by binding to the substrate and inducing a conformation change in the substrate (3), although interaction between PCPE‑1 and full‑length BMP‑1/tolloid proteinases has also been reported (4).

References

  1. Steiglitz, B.M. et al. (2002) J. Biol. Chem. 277:49820.
  2. Kessler, E. et al. (1990) Biochem. Biophys. Res. Commun. 173:81.
  3. Moali, C. et al. (2005) J. Biol. Chem. 280:24188.
  4. Ge, G. et al. (2006) J. Biol. Chem. 281:10786.

Long Name

Procollagen C-Proteinase Enhancer-1

Alternate Names

PCOLCE, PCPE1

Entrez Gene IDs

5118 (Human); 18542 (Mouse)

Gene Symbol

PCOLCE

UniProt

Q15113

Additional PCPE-1 Products

Product Documents for Recombinant Human PCPE-1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human PCPE-1 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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