Recombinant Human PD-L2 Fc Biotinylated Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT1224

R&D Systems, part of Bio-Techne
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BT1224-050
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Key Product Details

Source

NS0

Accession #

Q9BQ51

Structure / Form

Disulfide-linked homodimer

Conjugate

Biotin

Applications

Bioactivity

View all PD-L2/B7-DC Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human PD-L2/B7-DC protein
Human PD-L2
(Leu20-Pro219)
Accession # Q9BQ51		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20

Predicted Molecular Mass

49 kDa (unlabeled)

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is immobilized at 100 ng/mL, 100 μL/well, the concentration of Biotinylated Recombinant PD-L2 Fc Chimera that produces 50% of the optimal binding response is 0.04-0.24 μg/mL.

Scientific Data Images for Recombinant Human PD-L2 Fc Biotinylated Protein, CF

Recombinant Human PD-L2 Fc Biotinylated Protein Binding Activity

Recombinant Human PD-L2 Fc Biotinylated Protein Binding Activity

When Recombinant Human PD-1 Fc Chimera Recom-binant Human PD-1 Fc Chimera (Catalog # 1086-PD) is coated at 0.1 µg/mL, Biotinylated Recom-binant Human PD-L2 Fc Chimera (Catalog # BT1224) binds with an ED50 of 0.04-0.24 µg/mL.
Recombinant Human PD-L2 Fc Biotinylated Protein Flow Cytometry

Recombinant Human PD-L2 Fc Biotinylated Protein Flow Cytometry

In a functional flow cytometry test, (A) Recombinant Human PD-L2 Fc Biotinylated Protein (Catalog # BT1224) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-1 and EGFP. Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050), which does not stain the cells in the absence of recombinant protein (B).

Formulation, Preparation and Storage

BT1224
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L2/B7-DC

Programmed Death Ligand 2 (PD-L2), also known as B7-DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). Mature human PD-L2 consists of a 201 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2, 3). Within the ECD, mouse and human PD-L2 share 72% aa sequence identity. Alternative splicing generates additional isoforms that lack the second Ig-like domain and may be substituted and truncated following the first Ig-like domain (4). PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3, 5, 6). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (7-11). PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 12, 13). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (14). In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (9, 11).

References

  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
  3. Tseng, S.-Y. et al. (2001) J. Exp. Med. 193:839.
  4. He, X.-H. et al. (2004) Acta Biochim. Biophys. Sinica 36:284.
  5. Messal, N. et al. (2011) Mol. Immunol. 48:2214.
  6. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
  7. Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
  8. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
  9. Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
  10. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
  11. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
  12. Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
  13. Shin, T. et al. (2003) J. Exp. Med. 198:31.
  14. Xiao, Y. et al. (2014) J. Exp. Med. 211:943.

Long Name

Programmed Death-1 Ligand-2

Alternate Names

B7-DC, Butyrophilin-like Protein, CD273, PDCD1LG2, PDL2

Entrez Gene IDs

80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)

Gene Symbol

PDCD1LG2

UniProt

Q9BQ51

Additional PD-L2/B7-DC Products

Product Documents for Recombinant Human PD-L2 Fc Biotinylated Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human PD-L2 Fc Biotinylated Protein, CF

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