Recombinant Human PDGFRL Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1723-PR

R&D Systems, part of Bio-Techne
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1723-PR-050
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Key Product Details

Source

HEK293

Accession #

Q15198

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all PDGFRL Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human PDGFRL protein
Human PDGFRL
(Gln22-Ser375)
Accession # Q15198		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln22 inferred from enzymatic pyroglutamate treatment revealing His23.

Predicted Molecular Mass

68 kDa

SDS-PAGE

69-84 kDa, reducing conditions

Activity

Measured by its ability to inhibit proliferation of HCT-116 human colorectal carcinoma cells.
The ED50 for this effect is 1.5-9 μg/mL.

Scientific Data Images for Recombinant Human PDGFRL Fc Chimera Protein, CF

Recombinant Human PDGFRL Fc Chimera Protein Bioactivity

Recombinant Human PDGFRL Fc Chimera Protein Bioactivity

Recombinant Human PDGFRL Fc Chimera (Catalog # 1723-PR) inhibits HCT-116 human Colorectal carcinoma proliferation. The ED50 for this effect is 1.5-9 μg/mL.
Recombinant Human PDGFRL Fc Chimera Protein SDS-PAGE

Recombinant Human PDGFRL Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human PDGFRL Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 69- 84 kDa and 140- 170 kDa, respectively.

Formulation, Preparation and Storage

1723-PR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: PDGFRL

Platelet-derived growth factor receptor-like protein is a 67 kDa glycoprotein protein consisting of two Ig-like C2-type domains. By sequence similarity, human PDGFRL is 90% similar to the mouse version and 91% similar to that of the rat. It is a secreted protein related to the class III subfamily of receptor tyrosine kinases (RTK), the platelet-derived growth factor receptors (1-5). PDGFRL is a tumor suppressor active in the tumor-suppression network and implicated in colorectal cancer, and a decrease in PDGFRL expression levels has been observed in breast cancer (1). A variant of PDGFRL is found to play a role in the development of Behçet disease, a complex immunoregulatory disease (6). The autoimmune role of PDGFRL is also supported by its up-regulation in a mouse model for Rheumatoid Arthritis (7). Study also showed that PDGFRL may play a role in chondrocyte proliferation and differentiation.

References

  1. Guo, F-J. et al. (2010) World J Gastroenterol. 16:1465.
  2. Andrae, J. et al. (2008) Genes Dev. 22:1276.
  3. Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
  4. Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917.
  5. Matsui, T. et al. (1989) Science 243:800.
  6. Hou, S. et al. (2013). Human Mutat. 34:74.
  7. Fujikado, N. et al. (2006). Arthritis Res. & Ther. 8:R100.
  8. Kawata, K. et al. (2017) J Cell Biochemistry. 118:4033.

Long Name

Platelet-derived Growth Factor Receptor-like

Alternate Names

PDGFR-Like Protein, PDGRL, PRLTS

Entrez Gene IDs

5157 (Human); 68797 (Mouse); 290771 (Rat)

Gene Symbol

PDGFRL

UniProt

Q15198

Additional PDGFRL Products

Product Documents for Recombinant Human PDGFRL Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human PDGFRL Fc Chimera Protein, CF

For research use only

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