Recombinant Human PFKFB3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8566-BP

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
8566-BP-020
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Accession #

Q16875

Conjugate

Unconjugated

Applications

Enzyme Activity

View all PFKFB3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human PFKFB3 protein
Pro2-His520 with N-terminal Met and 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

60 kDa

SDS-PAGE

57 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate fructose-6-phosphate.
The specific activity is >70 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8566-BP
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: PFKFB3

6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls the activity of the enzymes phosphofructokinase 1 (PFK-1) and fructose 1,6-bisphosphatase (FBPase-1) to regulate glycolysis and gluconeogenesis (1-3). PFKFB3 has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. Recently, it is reported to be involved in vessel sprouting of endothelial cells (ECs) (4). ECs rely on glycolysis rather than on oxidative phosphorylation for ATP production and that loss of the glycolytic activator PFKFB3 in ECs impairs vessel formation. PFKFB3 also plays a crucial role in the progression of cancerous cells by enabling their glycolytic pathways even under severe hypoxic conditions (5, 6), which makes it a potential target for cancer therapy (7). The kinase activity of recombinant human PFKFB3 was assayed using a phosphatase-coupled method (8).

References

  1. Wu, C. et al. (2006) Adv. Enzyme Regul. 46:72.
  2. Sakai, A. et al. (1996) J. Biochem. 119:506.
  3. Manzano A. et al. (1998) Cytogenet. Cell Genet. 83:214.
  4. De Bock, K. et al. (2013) Cell 154:651.
  5. Chesney, J. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96:3047.
  6. Marsin, A.S. et al. (2002) J. Bio. Chem. 277:30778.
  7. Kim, S.G. et al. (2006) J. Bio. Chem. 281:2939.
  8. Wu, Z.L. et al. (2011) PloS ONE 6(8):e23172.

Long Name

6-Phosphofructo-2-Kinase/ Fructose-2,6-Bisphosphatase

Alternate Names

6 Phosphofructo 2 Kinase, IPFK2, PFK/FBPase 3, PFK2

Entrez Gene IDs

5209 (Human); 170768 (Mouse); 117276 (Rat)

Gene Symbol

PFKFB3

UniProt

Q16875

Additional PFKFB3 Products

Product Documents for Recombinant Human PFKFB3 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human PFKFB3 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.