Recombinant Human PGLYRP3/PGRP-I alpha Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 8507-PG
Key Product Details
Source
CHO
Accession #
Structure / Form
Disulfide-linked Homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human PGLYRP3/PGRP-I alpha protein
Trp18-His341, with a C-terminal 6-His tag
Trp18-His341, with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Trp18
Predicted Molecular Mass
37 kDa
SDS-PAGE
34-40 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When peptidoglycan is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human PGLYRP3/PGRP-I alpha that produces 50% optimal binding response is 10-60 ng/mL.
When peptidoglycan is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human PGLYRP3/PGRP-I alpha that produces 50% optimal binding response is 10-60 ng/mL.
Formulation, Preparation and Storage
8507-PG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 500 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: PGLYRP3/PGRP-I alpha
PGLYRP3, also known as PGRP-1 alpha, is a member of the peptidoglycan recognition protein family of innate immunity proteins (1, 2). Human PGLYRP3 is expressed in the skin, eyes, salivary glands, throat, tongue, esophagus, stomach, and intestine (3). Mature human PGLYRP3 contains two nonidentical PGRP domains, and it shares 74% and 75% amino acid sequence identity with mouse and rat PGLYRP3, respectively (1, 4). It is secreted as disulfide-linked homodimers and binds peptidoglycan (PGN) and PGN-containing Gram-positive bacteria (1, 3). PGLYRP3 is directly cytotoxic against pathogenic and nonpathogenic Gram-positive and Gram-negative bacteria (3, 5). Its bactericidal activity requires physiological concentrations of Zn2+ (5). PGLYRP3 has also been shown to have an anti-inflammatory role in intestinal epithelial cells (6). PGLYRP3 knockout mice are more sensitive to the development of experimental dermatitis and DSS-induced colitis than wild type mice (7-9). In humans, PGLYRP3 single nucleotide polymorphisms have been associated with inflammatory bowel disease (10).
References
- Liu, C. et al. (2001) J. Biol. Chem. 276:34686.
- Park, S.Y. et al. (2011) PLoS One 6:e24961.
- Lu, X. et al. (2006) J. Biol. Chem. 281:5895.
- Royet, J. and R. Dziarski (2007) Nat. Rev. Microbiol. 5:264.
- Wang, M. et al. (2007) J. Immunol. 178:3116.
- Zenhom, M. et al. (2012) Immunobiology 217:412.
- Park, S.Y. et al. (2011) PLoS One 6:e24961.
- Saha, S. et al. (2010) Cell Host Microbe 8:147.
- Jing, X. et al. (2014) J. Immunol. 193:3055.
- Zulfiqar, F. et al. (2013) PLoS One 8:e67393.
Long Name
Peptidoglycan Recognition Protein Intermediate, Alpha/Peptidoglycan Recognition Protein 3
Alternate Names
PGRP-I alpha, PGRP1A, PGRPI alpha, PGRPIA
Gene Symbol
PGLYRP3
UniProt
Additional PGLYRP3/PGRP-I alpha Products
Product Documents for Recombinant Human PGLYRP3/PGRP-I alpha Protein, CF
Product Specific Notices for Recombinant Human PGLYRP3/PGRP-I alpha Protein, CF
For research use only
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