Recombinant Human PGPEP-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6278-CY

R&D Systems, part of Bio-Techne
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6278-CY-010
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Key Product Details

Source

E. coli

Accession #

Q9NXJ5

Conjugate

Unconjugated

Applications

Enzyme Activity

View all PGPEP-1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human PGPEP-1 protein
Met1-His209, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

24 kDa

SDS-PAGE

24-26 kDa, reducing conditions

Activity

Measured by the hydrolysis of pyroglutamyl-AMC.
The specific activity is >9,500 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6278-CY
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: PGPEP-1

Pyroglutamyl peptidase-1 (PGPEP-1) is an omega peptidase which removes pyroglutamyl residues from the amino termini of peptides and proteins (1). It is a cytosolic cysteine peptidase that is expressed in most cell types (2). The enzyme requires a thiol‑reducing agent for activity (3). PGPEP-1 is potentially involved in the inactivation of biologically active peptides that possess an amino terminal pyroglutamyl group (3). Examples of such peptides include neurotensin, luteinizing hormone releasing hormone, and thyrotropin-releasing hormone.

References

  1. Kilbane Z. et al. (2007) Mol. Cell. Biochem. 297:189.
  2. Cummins P.M. and B. O'Connor (1998) Biochim. Biophys. Acta. 1429:1.
  3. Dando P.M. et al. (2003) Protein Express. Purif. 28:111.

Long Name

Pyroglutamyl Aminopeptidase I

Alternate Names

PAP-I, PGPEP1

Entrez Gene IDs

54858 (Human)

Gene Symbol

PGPEP1

UniProt

Q9NXJ5

Additional PGPEP-1 Products

Product Documents for Recombinant Human PGPEP-1 Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human PGPEP-1 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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