Recombinant Human PKLR Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8569-PK

Pyruvate Kinase Isozymes L/R
R&D Systems, part of Bio-Techne
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8569-PK-050
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Key Product Details

Source

E. coli

Accession #

P30613

Conjugate

Unconjugated

Applications

Enzyme Activity

View all PKLR Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human PKLR protein
Leu47-Ser574, with N-terminal Met and 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

58 kDa

SDS-PAGE

51-60 kDa, reducing conditions

Activity

Measured by its ability to transfer phosphate from phospho(enol)pyruvic acid monosodium salt hydrate (PEP) to adenosine 5'-diphosphate sodium salt (ADP).
The specific activity is >25,000 pmol/min/ug, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 8569-PK in the following applications:

Formulation, Preparation and Storage

8569-PK
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: PKLR

Pyruvate kinases (PK) are glycolytic enzymes that catalyze the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP, the final step in the glycolysis pathway (1, 2). There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is the major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues. R and L types are encoded in a same gene and caused by different splicing (3). The 33 amino acids from the N-terminus in the R type are placed with Met and Glu in the L type. PKLR is allosterically activated by fructose
1,6-bisphosphate (4). Mutation of PKLR is a frequent cause of hereditary non-spherocytic hemolytic anemia (5-8).

References

  1. Kanno, H. et al. (1991) Proc. Natl. Acad. Sci. U.S.A. 88:8218.
  2. Tani, K. et al. (1988) Proc. Natl. Acad. Sci. U.S.A. 85:1792.
  3. Kanno, H. et al. (1992) Biochem. Biophys. Res. Commun. 188:516.
  4. Valentini G. et al. (2002) J. Biol. Chem. 277:23807.
  5. Baronciani, L. and Beutler, E. (1993) Proc. Natl. Acad. Sci. U.S.A. 90:4324.
  6. Beutler E. and Baronciani L. (1996) Hum. Mutat. 7:1.
  7. Baronciani, L. et al. (1996) Blood Cells Mol. Dis. 22:259.
  8. Van Wijk, R. et al. (2009) Hum. Mutat. 30:446.

Long Name

Pyruvate Kinase, Liver And RBC

Alternate Names

PK1, PKL, Pyruvate Kinase 1, RPK

Entrez Gene IDs

5313 (Human); 18770 (Mouse)

Gene Symbol

PKLR

UniProt

P30613

Additional PKLR Products

Product Documents for Recombinant Human PKLR Protein, CF

Product Datasheets

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human PKLR Protein, CF

For research use only

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