Recombinant Human PLA2G4A Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6659-PL

R&D Systems, part of Bio-Techne
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6659-PL-010
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

NP_077734

Conjugate

Unconjugated

Applications

Enzyme Activity

View all PLA2G4A Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human PLA2G4A protein
Met1-Ala749, with a C-terminal 6-His tag

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2

Predicted Molecular Mass

86 kDa

SDS-PAGE

85-95 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze 1-Hexadecanoyl-2-(1-pyrene-decanoyl)-sn-glycero-3-phosphocholine.
The specific activity is >15 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6659-PL
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, TCEP,Brij-35 and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: PLA2G4A

Cytosolic Phospholipase A2a (PLA2G4A) belongs to the group IV phospholipase A2 family (1). These enzymes hydrolyze the ester bond at the second position (sn-2) of membrane glycerophospholipids. The group IV phospholipase A2 (PLA2G4) family is comprised of six intracellular enzymes (2). PLA2G4A has a preference for arachidonic acid in the sn-2 position of phospholipids indicating its involvement in the metabolism of eicosanoids. Its active site is characterized by a serine and aspartic acid dyad. The overall structural feature displays two domains, a lipid binding C2 domain and a catalytic a/b hydrolase domain. PLA2G4A translocation to the membrane and activation is facilitated by a calcium‑ion dependent conformational change in its C2 domain. In addition, phosphorylation of serine residues in PLA2G4A by protein kinases may be another regulatory mechanism for promoting the release of arachidonic acid. The knockout mouse studies of this enzyme indicate that the enzyme may play a major role in inflammatory diseases. Genetic ablation or reduction of PLA2G4A protected human amyloid precursor protein (APP) related pathogenesis from a mouse model (3). Mouse model studies of brain and lung cancer indicates that PLA2G4A may play a key regulatory role in angiogenesis of these tumors (4).

References

  1. Burke, J. E. and E.A. Dennis (2009) J. Lipid Res. 50:S237.
  2. Ghosh, M. et al. (2006) Prog. Lipid Res. 45:487.
  3. Sanchez-Mejia, R.O. et al. (2008) Nat. Neuroscience 11:1311.
  4. Linkous, A.G. et al. (2010) J. Natl. Cancer Inst. 102:1398.

Long Name

Phospholipase A2 Group IV A

Alternate Names

cPLA2, cPLA2-alpha

Entrez Gene IDs

5321 (Human); 18783 (Mouse); 24653 (Rat)

Gene Symbol

PLA2G4A

UniProt

NP_077734

Additional PLA2G4A Products

Product Documents for Recombinant Human PLA2G4A Protein, CF

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Product Specific Notices for Recombinant Human PLA2G4A Protein, CF

For research use only

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