Recombinant Human Plasma Kallikrein/KLKB1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2497-SE

R&D Systems, part of Bio-Techne
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2497-SE-010
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Key Product Details

Source

NS0

Accession #

P03952

Structure / Form

Pro and processed forms

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Plasma Kallikrein/KLKB1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Plasma Kallikrein/KLKB1 protein
Gly20-Ala638, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly20

Predicted Molecular Mass

70 kDa

SDS-PAGE

80 kDa, reducing conditions

Activity

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC).
The specific activity is >1,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

2497-SE
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Plasma Kallikrein/KLKB1

Human Plasma Kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma by binding to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase. Thus, it plays an important role in blood pressure regulation, fibrinolysis, and neutrophil activation (1). Human Plasma Kallikrein precursor contains a signal peptide (residues 1 to 19) and a pro form sequence (residues 20 to 638). Upon activation, the pro form is converted to a heavy chain and a light chain, which is linked by disulfide bonds and the latter contains the catalytic domain (2). The human Plasma Kallikrein pro form was expressed in the NS0 cells with a foreign signal peptide. The purified enzyme is mainly the pro form. When activated by thermolysin, it displays activity against a fluorogenic peptide substrate as described in Activity Assay Protocol. This activity can be inhibited by Human Serpin G1/C1 Inhibitor (Catalog # http://www.rndsystems.com/product_results.aspx?k=2488-PI">2488-PI).

References

  1. Coleman, R. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. (eds.) p. 1644, Academic Press, San Diego.
  2. Chung, N.W. et al. (1986) Biochemistry 25:2410.

Alternate Names

KLKB1, Plasma Prekallikrein

Entrez Gene IDs

3818 (Human); 16621 (Mouse)

Gene Symbol

KLKB1

UniProt

P03952

Additional Plasma Kallikrein/KLKB1 Products

Product Documents for Recombinant Human Plasma Kallikrein/KLKB1 Protein, CF

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Product Specific Notices for Recombinant Human Plasma Kallikrein/KLKB1 Protein, CF

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