Recombinant Human POGLUT1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6437-GT
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Protein O-Glucosyltransferase 1/POGLUT1 protein
Met1-Leu388, with a C-terminal 6-His tag
Accession # Q8NBL1
Met1-Leu388, with a C-terminal 6-His tag
Accession # Q8NBL1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg24 and Thr66
Predicted Molecular Mass
44 kDa
SDS-PAGE
40-60 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze UDP-Glucose.
The specific activity is >2 pmol/min/μg, as measured under the described conditions.
The specific activity is >2 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
6437-GT
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Protein O-Glucosyltransferase 1/POGLUT1
O-Glucosyltransferase1 (POGLUT1) is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase, that adds glucose to serine residues within the consensus sequence of C1‑X‑S‑X‑P‑C2 in Notch EGF repeats, thereby regulating cell-fate decisions (1). It is also known as CAP10-like protein (2) and KTELC1 due to the highly conserved CAP10 domain and the presence of an ER-retaining motif, KTEL, at the C‑terminus. The human gene is reported to be involved in the pathogenesis of both acute myelogenous and T‑acute lymphoblastic leukemias (3). Recently, POGLUT1 has been demonstrated in a phosphatase-coupled glycosyltransferase assay to have hydrolase activity on UDP-Glc (4).
References
- Acar, M. et al. (2008) Cell 132:247.
- Teng, Y. et al. (2006) Gene 371:7
- Wang, Y. et al. (2010) Genet Test Mol Biomarkers 14:127.
- Wu, Z.L. et al. (2010) Glycobiology, in press.
Alternate Names
CLP46, KDELCL1, KTELC1, MDS010, MDSRP, POGLUT1, Rumi
Entrez Gene IDs
56983 (Human)
Gene Symbol
POGLUT1
UniProt
Additional Protein O-Glucosyltransferase 1/POGLUT1 Products
Product Documents for Recombinant Human POGLUT1 Protein, CF
Product Specific Notices for Recombinant Human POGLUT1 Protein, CF
Coomassie is a registered trademark of Imperial Chemical Industries Ltd.
For research use only
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