Recombinant Human PPA1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6557-PP

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
6557-PP-010
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Accession #

Q15181

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Inorganic Pyrophosphatase/PPA1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Inorganic Pyrophosphatase/PPA1 protein
Met1-Asn289, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2

Predicted Molecular Mass

33 kDa

SDS-PAGE

34-36 kDa

Activity

Measured by its ability to hydrolyze pyrophosphate.
The specific activity is >50,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6557-PP
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Inorganic Pyrophosphatase/PPA1

Inorganic Pyrophosphatases are enzymes that catalyze the hydrolysis of pyrophosphate to two phosphate ions (1). This reaction is highly exergonic, and is utilized in many biochemical pathways, such as DNA synthesis (2) and bone formation (3), to render reactions effectively irreversible (4, 5). Likewise, inorganic pyrophosphatases can be coupled to unfavorable biochemical reactions in order to drive these reactions to completion, such as in the synthesis of activated sulfur donor 3’‑phosphoadenosine-5’-phosphosulfate (PAPS) (6). Two inorganic pyrophosphatases, i.e. PPA1 and PPA2, are found in humans. PPA1 is highly active on pyrophosphate and is ubiquitously expressed (7).

References

  1. Harold, FM (1966) Bacteriol. Rev. 30:772.
  2. Nelson, D.L. and Cox, M.M. (2000) Lehninger Principles of Biochemistry, 3rd ed. pp.937. ISBN 1-57259.
  3. Poole, K.E. and Reeve, J. (2005) Curr. Opin. Pharmacol. 5:612.
  4. Takahashi, K. et al. (2004) Biochem. Biophys. Res. Commun. 325:203.
  5. Terkeltaub, R.A. (2001) Am. J. Physiol., Cell Physiol. 281:C1.
  6. Wu, Z.L. et al. (2010) BMC Biotechnology 10:11.
  7. Fairchild, T.A. and Patejunas, G. (1999) Biochim. Biophys. Acta 1447:133.

Alternate Names

IOPPP, IPP1, PP1, PPase, SID6-8061

Entrez Gene IDs

5464 (Human); 852296 (Yeast)

Gene Symbol

PPA1

UniProt

Q15181

Additional Inorganic Pyrophosphatase/PPA1 Products

Product Documents for Recombinant Human PPA1 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human PPA1 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.