Recombinant Human PRL-3/PTP4A3 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 8455-PT
Key Product Details
Product Specifications
Source
E. coli-derived human PRL-3/PTP4A3 protein
Ala2-Met173, with N-terminal Met and 7-His tag
Ala2-Met173, with N-terminal Met and 7-His tag
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
20 kDa
SDS-PAGE
23 kDa, reducing conditions
Activity
Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
The specific activity is >0.4 pmol/min/μg, as measured under the described conditions.
The specific activity is >0.4 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
8455-PT
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: PRL-3/PTP4A3
Phosphatase of regenerating liver 3 (PRL-3), also known as protein-tyrosine phosphatase 4A3 (PTP4A3), is a member of the PRL subgroup of PTPases (1). It is preferentially expressed in skeletal muscle and the heart (2). Human PRL-3 shares 97% amino acid sequence identity with mouse and rat PRL-3. Structurally, PRL-3 consists of a five-stranded beta-sheet and six alpha-helices (3, 4). It has been shown to associate with membranes in a farnesylation-dependent manner (5). Both PRL-3 over-expression and attenuation of PRL-3 expression results in cell cycle arrest, suggesting that basal expression levels of this enzyme are important for normal cell cycle progression (6). PRL-3 has been shown to activate NF-kappa B signaling and may itself be regulated by FKBP38 (7, 8). PRL-3 also promotes epithelial to mesenchymal transition, tumor angiogenesis, cell migration, invasion, and metastasis, and it is over-expressed in multiple human cancers (9-18). Src-mediated phosphorylation of PRL-3 may be required for PRL-3-dependent cell migration and invasion (19).
References
- Bessette, D.C. et al. (2008) Cancer Metastasis Rev. 27:231.
- Zeng, Q. et al. (1998) Biochem. Biophys. Res. Commun. 244:421.
- Kozlov, G. et al. (2004) J. Biol. Chem. 279:11882.
- Kim, K.A. et al. (2004) FEBS Lett. 565:181.
- Zeng, Q. et al. (2000) J. Biol. Chem. 275:21444.
- Basak, S. et al. (2008) Mol. Cell 30:303.
- Lian, S. et al. (2013) Biochem. Biophys. Res. Commun. 430:196.
- Choi, M.S. et al. (2011) Biochem. Biophys. Res. Commun. 406:305.
- Wang, H. et al. (2007) Cancer Res. 67:2922.
- Liu, Y. et al. (2009) Cancer Biol. Ther. 8:1352.
- Pryczynicz, A. et al. (2014) Tumour Biol. 35:6587.
- Guo, K. et al. (2006) Cancer Res. 66:9625.
- Ming, J. et al. (2014) Pathobiology 81:1.
- Zeng, Q. et al. (2003) Cancer Res. 63:2716.
- Kato, H. et al. (2004) Clin. Cancer Res. 10:7318.
- Al-Aidaroos, A.Q. and Q. Zeng (2010) J. Cell. Biochem. 111:1087.
- Jiang, Y. et al. (2011) Cancer Res. 71:234.
- Krndija, D. et al. (2012) J. Cell Sci. 125:3883.
- Fiordalisi, J.J. et al. (2013) PLoS One 8:e64309.
Long Name
Phosphatase of Regenerating Liver 3
Alternate Names
PRL3, PTP4A3
Gene Symbol
PTP4A3
UniProt
Additional PRL-3/PTP4A3 Products
Product Documents for Recombinant Human PRL-3/PTP4A3 Protein, CF
Product Specific Notices for Recombinant Human PRL-3/PTP4A3 Protein, CF
For research use only
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