Recombinant Human Procalcitonin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9607-PN

R&D Systems, part of Bio-Techne
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9607-PN-050
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Key Product Details

Source

E. coli

Accession #

P01258-1

Conjugate

Unconjugated

View all Procalcitonin Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Procalcitonin protein
Pro27-Asn141, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro27

Predicted Molecular Mass

14 kDa

SDS-PAGE

14 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9607-PN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Procalcitonin

Procalcitonin (PCT) is encoded by a single CALCA gene, and can result in 2 alternatively spliced products: calcitonin and calcitonin-gene related peptide (CGRP) (1). Mature PCT is expressed as a 116 amino acid (aa) protein which is subsequently cleaved into 3 parts: a 57 aa pro-region, a 32 aa Calcitonin peptide and a 21 aa Katacalcin peptide (2, 3). PCT is moderately conserved among species, with human sharing 72% and 75% sequence identity with mouse and rat, respectively. In healthy adults, PCT is expressed by thyroid C cells and promptly converted to calcitonin (3). Calcitonin is directly involved in osteoclast activity and bone resorption (4). However, in states of inflammatory disease or bacterial infection, PCT is over-expressed from multiple cell types and the levels of unprocessed PCT rise significantly (3-5). These increased levels of PCT in circulation have been used as a diagnostic marker of systemic bacterial infection and sepsis and as a guide to antibiotic therapy (5, 6). More recently, the use of PCT as a disease marker has been expanded to include acute heart failure and vasculitic disorders (7, 8).

References

  1. Russwurm, S. et al. (2005). Clin Chem and Lab Med, 37:789.
  2. Le Moullec, J.M. et al. (1984) FEBS Lett 167:93.
  3. Maruna P. et al. (2000) Physiol Res;49 Suppl 1:S57.
  4. Becker, KL et al. (2004). J Clin Endocrinol Metab 89:1512.
  5. Müller B. et al. (2001) Jan;86(1):396-404. J Clin Endocrinol Metab.
  6. Meisner, M. et al. (2014) Ann Lab Med. Jul; 34:263.
  7. Möckel, M. et al. (2017) ESC Heart Failure, 4:203.
  8. Lee NH, et al. (2017) Korean J of Peds 60:112.

Alternate Names

CALC1, CALCA, Calcitonin 1, calcitonin gene-related peptide 1, CGRP, Katacalcin

Entrez Gene IDs

796 (Human); 12310 (Mouse); 24241 (Rat)

Gene Symbol

CALCA

UniProt

P01258-1

Additional Procalcitonin Products

Product Documents for Recombinant Human Procalcitonin Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Procalcitonin Protein, CF

For research use only

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