Recombinant Human Properdin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8216-PR

R&D Systems, part of Bio-Techne
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8216-PR-050
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Key Product Details

Source

HEK293

Accession #

P27918

Conjugate

Unconjugated

Applications

Bioactivity

View all Properdin Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Properdin protein
Asp28-Leu469, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp28

Predicted Molecular Mass

50 kDa

SDS-PAGE

57-63 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human Properdin binds to Zymosan with a ED50 of ≤1.25 µg/mL.

Formulation, Preparation and Storage

8216-PR
Formulation Supplied as a 0.2 μm filtered solution in Sodium Citrate and NaCl.
Reconstitution

Reconstitution Buffer Available:
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Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Properdin

Properdin, also known as Complement Factor P, is an approximately 53 kDa glycoprotein that positively regulates the complement alternative pathway (AP) (1, 2). It consists of seven thrombospondin repeat (TSR) domains, referred to as TSR0-TSR6 beginning at the N-terminus (3). Under physiological conditions, Properdin can form cyclic dimers, trimers, and tetramers through head-to-tail interactions of monomeric subunits (4-6). Non-physiological high molecular weight polymers of Properdin can also form during long term storage and freeze/thaw cycles (5, 7). Mature human Properdin shares 79% and 77% amino acid sequence identity with rat and mouse Properdin, respectively. Interestingly, human Properdin has been shown to restore LPS-dependent AP complement activity in Properdin-/- mouse serum (8). Properdin may also act as a pattern-recognition molecule, since it binds glycosaminoglycan (GAG) chains of proteoglycans on apoptotic T cells to promote complement activation and phagocytosis (9). Furthermore, Properdin has been shown to bind certain microbial targets and serve as a platform for C3bBbP assembly and function (10, 11). Underscoring its importance to immunity, Properdin deficiency is associated with significant risk of meningococcal disease in humans (12). It may also be involved in the development of abdominal aortic aneurysm, an inflammatory vascular disease (13).

References

  1. Pillemer, L. et al. (1954) Science 120:279.
  2. Kemper, C. et al. (2010) Annu. Rev. Immunol. 28:131.
  3. Alcorlo, M. et al. (2013) Proc. Natl. Acad. Sci. USA 110:13504.
  4. Smith, C.A. et al. (1984) J. Biol. Chem. 259:4582.
  5. Pangburn, M.K. (1989) J. Immunol. 142:202.
  6. Cortes, C. et al. (2013) Front. Immunol. 3:412.
  7. Farries, T.C. et al. (1987) Biochem. J. 243:507.
  8. Kimura, Y. et al. (2008) Blood 111:732.
  9. Kemper, C. et al. (2008) Proc. Natl. Acad. Sci. USA 105:9023.
  10. Hourcade, D.E. (2006) J. Biol. Chem. 281:2128.
  11. Spitzer, D. et al. (2007) J. Immunol. 179:2600.
  12. Linton, S.M. and B.P. Morgan (1999) Clin. Exp. Immunol. 118:189.
  13. Zhou, H.F. et al. (2012) Proc. Natl. Acad. Sci. USA 109:E415.

Alternate Names

CFP, Complement Factor P, PFC

Entrez Gene IDs

5199 (Human); 18636 (Mouse); 299314 (Rat)

Gene Symbol

CFP

UniProt

P27918

Additional Properdin Products

Product Documents for Recombinant Human Properdin Protein, CF

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Product Specific Notices for Recombinant Human Properdin Protein, CF

For research use only

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