Recombinant Human PTGES2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7627-PS

R&D Systems, part of Bio-Techne
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7627-PS-020
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Key Product Details

Source

E. coli

Accession #

Q9H7Z7

Conjugate

Unconjugated

View all Prostaglandin E Synthase 2/PTGES2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Prostaglandin E Synthase 2/PTGES2 protein
Glu88-His377, with an N-terminal Met and 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

34 kDa

SDS-PAGE

28-32 kDa, reducing conditions

Formulation, Preparation and Storage

7627-PS
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Prostaglandin E Synthase 2/PTGES2

PTGES2, also known as membrane-associated prostaglandin E synthase (mPGES) catalyzes the conversion of prostaglandin H2 (PGH2) to prostaglandin E2 (PGE2). Its N-terminal domain anchors it in the Golgi membrane, and its catalytic domain resides in the cytoplasm. Proteolysis within the cell generates a truncated soluble form beginning with amino acid 88 that resides in the cytosol (1). PTGES2 production of PGE2 is coupled to both COX-1 and COX-2 (2). PTGES2 is expressed in many tissues, with highest expression in brain, heart, skeletal muscle, kidney, and liver (1). In addition to its PGE2 production, PTGES2 activates transcription regulated by a gamma-interferon-activated transcription element (3). The protein may also play a role in colorectal cancer progression (4).

References

  1. Tanikawa, N. et al. (2002) Biochem. Biophys. Res. Commun. 291:884.
  2. Murakami, M. et al. (2003) J. Biol. Chem. 278:37937.
  3. Hu, J. et al. (2002) J. Biol. Chem. 277:30253.
  4. Seo, T. et al. (2009) Virchows Arch. 454:667.

Alternate Names

C9orf15, GBF1, mPGES2, PGES2

Entrez Gene IDs

80142 (Human); 96979 (Mouse); 311865 (Rat)

Gene Symbol

PTGES2

UniProt

Q9H7Z7 (Human)

Additional Prostaglandin E Synthase 2/PTGES2 Products

Product Documents for Recombinant Human PTGES2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human PTGES2 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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