Recombinant Human PTPR sigma Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3430-PR

R&D Systems, part of Bio-Techne
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3430-PR-050
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Key Product Details

Source

NS0

Accession #

NP_570924

Conjugate

Unconjugated

Applications

Bioactivity

View all PTP sigma/PTPRS Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human PTP sigma/PTPRS protein
Glu30-Gly1260, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu30 & Ser1173

Predicted Molecular Mass

135.8 kDa

SDS-PAGE

120-145 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PTPR sigma/PTPRS  is coated at 2 μg/mL, Recombinant Human TrkC Fc Chimera (Catalog # 373‑TC) binds with an apparent Kd <1 nM.

Formulation, Preparation and Storage

3430-PR
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTP sigma/PTPRS

Phosphatase Receptor-type sigma (PTPR sigma), also known as PTP sigma, is a 200 kDa type I transmembrane glycoprotein that is a member of the class 2A (LAR) subfamily of the protein tyrosine phosphatase (PTP) family (1). Human PTPR sigma cDNA encodes 1948 amino acids (aa) including a 29 aa signal sequence, a 1253 aa extracellular domain (ECD) with three C2-type Ig domains and eight Fibronectin (Fn) type III domains, a transmembrane domain, and a cytoplasmic region with two PTP domains (1, 2). Multiple differentially expressed splice variants lack sections of the Ig and/or Fn domains and/or cytoplasmic juxtamembrane sequence (1, 2). PKC activation in the cell promotes endoprotease cleavage of PTPR sigma, releasing the 140-160 kDa ECD (3). The human PTPR sigma ECD shares 93% aa sequence identity with the nearest isoform of mouse and rat PTPR sigma, 96% with bovine PTPR sigma, and 97% with porcine PTPR sigma. Activity of PTPR sigma can be inhibited by bisphosphonates (2). PTPR sigma is a receptor for several proteins, including neural chondroitin sulfate proteoglycans which inhibit nerve regeneration (4, 5). It also binds and dephosphorylates targets such as N-Cadherin, E-Cadherin, EGF R and Ret (6-9). Through these interactions PTPR sigma affects pituitary and neuroendocrine development, and axon growth and targeting (4-6). Mice deleted for PTPR sigma show pituitary, pancreatic, and gut abnormalities, mainly due to growth hormone insufficiency (9-12). They also show enhanced regeneration, but faulty targeting, of injured peripheral nerves (4-6, 13). Head and neck squamous cell cancers frequently underexpress PTPR sigma (14). Since PTPR sigma inhibits EGF R signaling pathways, its underexpression can enhance tumor cell growth (14).

References

  1. Pulido, R. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11686.
  2. Endo, N. et al. (1996) J. Bone Miner. Res. 11:535.
  3. Aicher, B. et al. (1997) J. Cell Biol. 138:681.
  4. Shen, Y. et al. (2009) Science 326:592.
  5. Duan, Y. and R.J. Giger (2010) Sci. Signal. 3:pe6.
  6. Siu, R. et al. (2007) Mol. Cell. Biol. 27:208.
  7. Vijayvargia, R. et al. (2004) Biochem. Biophys. Res. Commun. 325:344.
  8. Uetani, N. et al. (2009) J. Clin. Invest. 119:924.
  9. Muise, A.M. et al. (2007) Curr. Biol. 17:1212.
  10. Elchebly, M. et al. (1999) Nat. Genet. 21:330.
  11. Batt, J. et al. (2002) Mol. Endocrinol. 16:155.
  12. Chagnon, M.J. et al. (2006) Can. J. Physiol. Pharmacol. 84:755.
  13. McLean, J. et al. (2002) J. Neurosci. 22:5481.
  14. Morris, L.G. et al. (2011) Proc. Natl. Acad. Sci. USA 108:19024.

Long Name

Protein Tyrosine Phosphatase Receptor-type Sigma

Alternate Names

PTPR sigma, PTPRS

Entrez Gene IDs

5802 (Human); 19280 (Mouse)

Gene Symbol

PTPRS

UniProt

NP_570924

Additional PTP sigma/PTPRS Products

Product Documents for Recombinant Human PTPR sigma Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human PTPR sigma Protein, CF

For research use only

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