Recombinant Human PVRIG Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9365-PV

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

Q6DKI7

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all PVRIG Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human PVRIG protein
Human PVRIG
(Thr41 & Glu43-Leu172)
Accession # Q6DKI7		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr41 & Glu43

Predicted Molecular Mass

40 kDa

SDS-PAGE

51-57 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PVRIG Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human
Nectin-2/CD112 (Catalog # 2229-N2) that produces 50% of the optimal binding response is 0.4-2.4 μg/mL.

Scientific Data Images for Recombinant Human PVRIG Fc Chimera Protein, CF

Recombinant Human PVRIG Fc Chimera Protein Bioactivity

Recombinant Human PVRIG Fc Chimera Protein Bioactivity

When Recombinant Human PVRIG Fc Chimera (Catalog # 9365-PV) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human Nectin-2/CD112 (Catalog # 2229-N2) binds with an ED50 of 0.4-2.4 µg/mL.

Formulation, Preparation and Storage

9365-PV
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and PEG with Trehalose.
Reconstitution
Reconstitute at 250 μg/mL in sterile water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PVRIG

Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family (1). It is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. The intracellular domain contains two tyrosine residues, one within an ITIM-like motif that is a potential docking site for phosphatases (1). The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. The human PVRIG gene is preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells (1). PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells (1).

References

  1. Zhu, Y., et.al. (2016) J. Exp. Med. 213:167.

Long Name

Poliovirus Receptor Related Immunoglobulin Domain Containing

Alternate Names

C7orf15, CD112R

Entrez Gene IDs

79037 (Human); 102640920 (Mouse); 102127364 (Cynomolgus Monkey)

Gene Symbol

PVRIG

UniProt

Q6DKI7

Additional PVRIG Products

Product Documents for Recombinant Human PVRIG Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human PVRIG Fc Chimera Protein, CF

For research use only

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