Recombinant Human QPCT Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6368-ZN

R&D Systems, part of Bio-Techne
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6368-ZN-010
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q16769

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Glutaminyl-peptide Cyclotransferase/QPCT Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Glutaminyl-peptide Cyclotransferase/QPCT protein
Ala33-Leu361, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

38 kDa

SDS-PAGE

36-40 kDa, reducing conditions

Activity

Measured by its ability to convert Glutaminyl-AMC to pyroglutamyl-AMC.
The specific activity is >550 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6368-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Glutaminyl-peptide Cyclotransferase/QPCT

Glutaminyl-peptide Cyclotransferase, also known as Glutaminyl Cyclase, catalyzes the conversion of N‑terminal L-glutaminyl residues of peptides to pyroglutamyl groups (1). The enzyme is present in the pituitary and adrenal glands, where it is important for the generation of the N‑terminal pyroglutamyl groups of peptide hormones such as neurotensin and thyrotropin-releasing hormone. Glutaminyl Cyclase also catalyzes the conversion of N‑terminal L‑glutamyl residues to pyroglutamyl residues (2). This activity may contribute to the formation of several amyloid-related plaque forming peptides, contributing to Alzheimer’s disease pathology. Glutaminyl Cyclase is also considered to be a diagnostic marker of thyroid tumors (3).

References

  1. Busby, W.H. Jr. et al. (1987) J. Biol. Chem. 262:8532.
  2. Schilling, S. et al. (2004) FEBS Lett. 563:191.
  3. Griffith, O.L. et al. (2006) J. Clin. Oncol. 24:5043.

Alternate Names

GCT, Glutaminyl cyclase, Glutamyl cyclase

Entrez Gene IDs

25797 (Human)

Gene Symbol

QPCT

UniProt

Q16769

Additional Glutaminyl-peptide Cyclotransferase/QPCT Products

Product Documents for Recombinant Human QPCT Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human QPCT Protein, CF

For research use only

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