Recombinant Human R-Spondin 1 Biotinylated Protein

R&D Systems, part of Bio-Techne | Catalog # BT4645

R&D Systems, part of Bio-Techne
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Carrier Free
BT4645-025/CF
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With Carrier
BT4645-025
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Key Product Details

Source

CHO

Accession #

Q2MKA7

Structure / Form

Biotinylated protein via sugars

Conjugate

Biotin

Applications

Bioactivity

View all R-Spondin 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 1 protein
Arg31-Ala263

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser21 & Arg31

Predicted Molecular Mass

26 kDa (unlabeled)

Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
The ED50 for this effect is typically 5-30 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.

Scientific Data Images for Recombinant Human R-Spondin 1 Biotinylated Protein

Both Biotinylated Recombinant Human R-Spondin 1 (Catalog # BT4645) and unlabeled Recombinant Human R-Spondin 1 (Catalog # 4645-RS) induces activation of beta-catenin response in Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is 5-30 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The similarity in activity highlights that the biotinylated protein is fully functional.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: BT4645
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: BT4645/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: R-Spondin 1

R-Spondin 1 (RSPO1), also known as Cristin 3, is a 27 kDa secreted protein in the R-Spondin family of Wnt/ beta-catenin signaling regulators (1). These proteins contain two adjacent cysteine-rich furin-like domains followed by a thrombospondin (TSP-1) motif and a region rich in basic residues. Mature human R-Spondin 1 shares 87% amino acid sequence identity with mouse and rat R-Spondin 1 (2). Alternative splicing generates additional isoforms that have a substituted N-terminus or lack the TSP-1 domain. R-Spondin 1 enhances canonical Wnt/ beta-catenin signaling by competing with the Wnt antagonist Dkk-1, binding to Frizzled-8, Kremen, LRP-6, Lgr4, Lgr5, and Lgr6, and enhancing cell surface availability of Wnt receptors (3-11). R-Spondin 1 functions in dorsal neural tube development (12) as well as male and female germ cell development (7, 10, 13). It also induces bone formation (6), intestinal crypt cell proliferation (14), angiogenesis (7, 15), and insulin secretion from pancreatic beta cells (11).

References

  1. Jin, Y.R. and J.K. Yoon (2012) Int. J. Biochem. Cell Biol. 44:2278.
  2. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
  3. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  4. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  5. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  6. Kronke, G. et al. (2010) Arthritis Rheum. 62:2303.
  7. Caruso, M. et al. (2015) PLoS One 10:e0124213.
  8. Hao, H.-X. et al. (2012) Nature 485:195.
  9. de Lau, W. et al. (2011) Nature 476:293.
  10. Chassot, A.-A. et al. (2011) PLoS One 6:e25641.
  11. Wong, V.S.C et al. (2010) J. Biol. Chem. 285:21292.
  12. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
  13. Chassost, A.-A. et al. (2012) Development 139:4461.
  14. Kim, K.-A. et al. (2005) Science 309:1256.
  15. Gore, A.V. et al. (2011) Development 138:4875.

Long Name

Roof Plate-specific Spondin 1

Alternate Names

Cristin 3, CRISTIN3, HRspo1, RSPO, RSPO1, RSpondin 1

Entrez Gene IDs

284654 (Human); 192199 (Mouse); 102122369 (Cynomolgus Monkey)

Gene Symbol

RSPO1

UniProt

Q2MKA7 (Human)

Additional R-Spondin 1 Products

Product Documents for Recombinant Human R-Spondin 1 Biotinylated Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS BT4645 (with carrier)
Download SDS BT4645/CF (carrier free)

Product Specific Notices for Recombinant Human R-Spondin 1 Biotinylated Protein

For research use only

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