Recombinant Human Relaxin-2 (B-33/A-24) Protein
R&D Systems, part of Bio-Techne | Catalog # 3596-RN
Key Product Details
Source
E. coli
Accession #
Structure / Form
Disulfide-linked heterodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
E. coli-derived human Relaxin-2 protein
Val23-Arg55 (B chain), with an N-terminal Met & Gln162-Cys185 (A chain)
Val23-Arg55 (B chain), with an N-terminal Met & Gln162-Cys185 (A chain)
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met (B chain) & Gln162 (A chain)
Predicted Molecular Mass
2.7 kDa (A chain), 3.9 kDa (B chain)
Activity
Measured by its ability to induce cAMP accumulation in THP-1 human acute monocytic leukemia cells. Parsell, D.A. et al. (1996) J. Biol. Chem. 271:27936.
The ED50 for this effect is 0.5-2.5 ng/mL.
The ED50 for this effect is 0.5-2.5 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 3596-RN
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 3596-RN/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Relaxin-2
Human Relaxin-2, also called H2 Relaxin, is a 6 kDa, 53 amino acid (aa) nonglycosylated, heterodimeric polypeptide that plays an important role in female reproduction (1, 2). Relaxin belongs to a structurally related insulin/relaxin superfamily that currently contains 10 members in human (2). To date, three human relaxin genes have been identified (1, 2). Among these, Relaxin-2 is best studied and the only known Relaxin to circulate in the blood (2, 3). As with other insulin/relaxin superfamily members, human Relaxin-2 is synthesized as a preprohormone (4). It is 18 kDa in size and 185 aa in length. It contains a 24 aa signal sequence, a 3.3 kDa, 31 aa B domain, a 106 aa C (or connecting) domain, and a C-terminal, 2.7 kDa, 24 aa A domain (2, 4, 5). Upon removal of the signal peptide, two intrachain disulfide bonds are created between the B and A chains. This is followed by prohormone convertase removal of the intervening C chain, creating a disulfide-linked heterodimer. Initially, the B chain is 31 aa in length and terminates with a Lys‑Arg dipeptide. This is subsequently cleaved by a carboxypeptidase to generate a 29 aa mature chain (5). The mature human Relaxin-2 heterodimer is 48%, 44% and 43% aa identical to rat, canine and porcine Relaxin-2, respectively. Human Relaxin-2 is 35% and 75% aa identical to human Relaxin-3 and 1, respectively. An alternate splice form for human Relaxin-2 has been reported (6). It is identical to the standard form through the first 70 aa of the preproprecursor. At this point, a 47 aa substitution occurs that appears to be absent in typical cleavage motifs. Relaxin confers its activity by binding to leucine-rich guanine nucleotide-binding (G-protein) coupled receptors, LGR7 and LGR8 (2, 7). Relaxin is best known as a hormone of parturition that promotes growth and softening of the cervix, and development of the mammary gland (2, 3). It also has a marked impact on the uterus. In particular, it promotes angiogenesis, inhibits MMP production and activity, and down‑regulates estrogen receptor-alpha expression (8).
References
- Hayes, E.S. (2004) Reprod Biol Endocrinol. 2:36.
- Sherwood, O.D. (2004) Endocr. Rev. 25:205.
- Wilkinson, T.N. et al. (2005) BMC Evol. Biol. 5:14.
- Hudson, P. et al. (1984) EMBO J. 3:2333.
- Marriott, D. et al. (1992) Mol. Endocrinol. 6:1441.
- Gunnersen, J.M. et al. (1996) Mol. Cell. Endocrinol. 118:85.
- Hsu, S.Y. et al. (2002) Science. 295:671.
- Goldsmith, L.T. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4685.
Alternate Names
Relaxin2, RLN2, RLXH2
Entrez Gene IDs
6019 (Human)
Gene Symbol
RLN2
UniProt
Additional Relaxin-2 Products
Product Documents for Recombinant Human Relaxin-2 (B-33/A-24) Protein
Product Specific Notices for Recombinant Human Relaxin-2 (B-33/A-24) Protein
For research use only
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