Recombinant Human Renin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4090-AS

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
4090-AS-020
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

P00797

Structure / Form

Pro form

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Renin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Renin protein
Leu24-Arg406, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu24

Predicted Molecular Mass

44 kDa

SDS-PAGE

44 kDa and 51 kDa, reducing conditions

Activity

Measured by its ability to cleave a fluorogenic peptide substrate, Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg.
The specific activity is >20 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

4090-AS
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Renin

Human Renin is a member of the aspartyl proteinase family produced largely in part by the juxtaglomerular cells in the kidney (1). Renin differs from the other members of this class by having a pH optimum near the neutral pH region with native substrates instead of a pH 2.0 to 3.4 range (2). This more neutral pH optimum allows it to be functional in the plasma. Renin also has a very high selectivity for substrates due to a long peptide recognition on either side of the peptide bond undergoing cleavage. An octapeptide substrate was the minimum length to be cleaved by Renin. Renin plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of Renin. Renin releases the decapeptide angiotensin I, which in turn is further converted to vasoactive hormone angiotensin II by angiotensin converting enzyme (ACE). Renin is produced as prorenin with 43 pro residues at the N‑terminal of mature Renin. The inactive prorenin becomes activated proteolytically by trypsin, cathepsin B, or other proteinases.

References

  1. Yokosawa, H. et al. (1980) J. Biol. Chem. 255:3498.
  2. Fuminaki, S. et al. (2004) in Handbook of Proteolytic Enzymes, Barret, A. J. et al. eds. p. 54.

Alternate Names

Angiotensinogenase, REN

Entrez Gene IDs

5972 (Human); 19701 (Mouse)

Gene Symbol

REN

UniProt

P00797

Additional Renin Products

Product Documents for Recombinant Human Renin Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Renin Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.