Recombinant Human ROR1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9490-RO

R&D Systems, part of Bio-Techne
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9490-RO-050
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Key Product Details

Source

HEK293

Accession #

Q01973-1

Structure / Form

Disulfide -linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all ROR1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ROR1 protein
Human ROR1
(Ala24-Glu403)
Accession # Q01973-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala24

Predicted Molecular Mass

69 kDa

SDS-PAGE

84-97 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Measured by its ability to bind biotinylated recombinant human/mouse Wnt-5a in a functional ELISA. The ED50 for this effect is 8-48 µg/mL.

Formulation, Preparation and Storage

9490-RO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR1

ROR1 (Receptor tyrosine kinase-like orphan receptor 1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1),  is a member of  the ROR family within receptor tyrosine kinases (RTK) superfamily. Two ROR family members (ROR1 and ROR2) have been identified and are characterized by the intracellular tyrosine kinase domains, highly related to those of the Trk-family receptor tyrosine kinases, and by the extracellular Frizzled-like cysteine-rich domains and kringle domains, which are common to receptors of the Wnt family members (1, 2). Human ROR1 is a type I transmembrane protein with 937 amino acids (aa) in length. It contains a 29 aa signal sequence, a 377 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 510 aa cytoplasmic region. Human ROR1 shares 97% and 58% aa sequence identity with mouse ROR1 and human ROR2, respectively (2, 3). ROR1 has been shown to play crucial roles in developmental morphogenesis by acting as receptors or co-receptors to mediate Wnt5a-induced signaling (4). The bioactivity of ROR1 is measured by its ability to bind biotinylated recombinant mouse Wnt-5a in a functional ELISA.

References

  1. Aghebati-Maleki, L. et al. (2017) Biomed. Pharmacother. 88:814.
  2. Borcherding N. et al. (2014) Protein Cell. 5:496.
  3. Masiakowski P. and Carroll R.D. (1992) J. Biol. Chem. 267:26181.
  4. Fukuda T. et al. (2008) Proc. Natl. Acad. Sci. U.S.A.  105:3047.

Long Name

Receptor Tyrosine Kinase-like Orphan Receptor 1

Alternate Names

NTRKR1

Entrez Gene IDs

4919 (Human); 26563 (Mouse)

Gene Symbol

ROR1

UniProt

Q01973-1

Additional ROR1 Products

Product Documents for Recombinant Human ROR1 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human ROR1 Fc Chimera Protein, CF

For research use only

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