Recombinant Human S100A11 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9015-S11

R&D Systems, part of Bio-Techne
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9015-S11-050
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Key Product Details

Source

E. coli

Accession #

P31949

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all S100A11 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human S100A11 protein
Ala2-Thr105

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

12 kDa

SDS-PAGE

9 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human ANXA-2 is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Human S100A11 that produces 50% of the optimal binding response is approximately 0.4-2.4 μg/mL.

Formulation, Preparation and Storage

9015-S11
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A11

S100A11, also known as S100C and Calgizzarin, is a 10 kDa member of the S100 family of calcium binding proteins (1-3). Human S100A11 contains two EF hand motifs and shares 82% amino acid sequence identity with mouse and rat S100A11. It forms covalent homodimers upon transglutamination and also disulfide-linked tetramers (4, 5). S100A11 is secreted by keratinocytes and can be crosslinked into the cornified envelope of the skin (6). Dimerization enhances its ability to signal through RAGE on keratinocytes, induce the production of EGF family proteins, and induce cell proliferation (7). Dimerization also enables S100A11 to bind RAGE on chondrocytes, leading to chondrocyte hypertrophy and catabolism of the cartilage matrix (4). S100A11 is additionally found in the cytosol where it becomes phosphorylated and translocates to the nucleus in response to DNA damage, RELM alpha exposure, or elevated extracellular calcium concentrations (8-10). Calcium also promotes S100A11 association with S100B as well as Annexins A1, A2, and A6 (5, 11-13). S100A11-Annexin A2 complexes are recruited to sites of plasma membrane damage where they facilitate membrane repair in migrating cancer cells (13). S100A11 is upregulated in various cancers and supports tumor cell proliferation, invasion, and migration (7, 14). In addition, S100A11 is produced in the ovary, and it acts on cumulus cells to inhibit oocyte fertilization (15).

References

  1. Donato, R. et al. (2013) Curr. Mol. Med. 13:24.
  2. Liu, Y. et al. (2015) Br. J. Pharmacol. 172:1664.
  3. Tanaka, M. et al. (1995) Cancer Lett. 89:195.
  4. Cecil, D.L. and R. Terkeltaub (2008) J. Immunol. 180:8378.
  5. Chang, N. et al. (2007) Am. J. Physiol. Cell Physiol. 292:C1417.
  6. Robinson, N.A. et al. (1997) J. Biol. Chem. 272:12035.
  7. Sakaguchi, M. et al. (2007) Mol. Biol. Cell 19:78.
  8. Gorsler, T. et al. (2010) BMC Cell Biol. 11:100.
  9. Fan, C. et al. (2011) Mol. Cell Proteomics 10:M110.000901.
  10. Sakaguchi, M. et al. (2003) J. Cell Biol. 163:825.
  11. Deloulme, J.C. et al. (2000) J. Biol. Chem. 275:35302.
  12. Mailliard, W.S. et al. (1996) J. Biol. Chem. 271:719.
  13. Jaiswal, J.K. et al. (2014) Nat. Commun. 5:3795.
  14. Liu, Y. et al. (2015) Exp. Ther. Med. 9:1460.
  15. Hanaue, M. et al. (2011) Mol. Reprod. Dev. 78:91.

Long Name

S100 Calcium Binding Protein A11

Alternate Names

Calgizzarin, MLN70, S100C

Entrez Gene IDs

6282 (Human); 20195 (Mouse); 445415 (Rat)

Gene Symbol

S100A11

UniProt

P31949

Additional S100A11 Products

Product Documents for Recombinant Human S100A11 Protein, CF

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Product Specific Notices for Recombinant Human S100A11 Protein, CF

For research use only

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