Recombinant Human SALM3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5445-SA

R&D Systems, part of Bio-Techne
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5445-SA-050
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Key Product Details

Source

CHO

Accession #

Q6PJG9

Conjugate

Unconjugated

Applications

Bioactivity

View all SALM3/LRFN4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human SALM3/LRFN4 protein
Cys17-Leu518 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys17

Predicted Molecular Mass

54.2 kDa

SDS-PAGE

70-90 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.
When 5 x 104 cells/well are added to Recombinant Human SALM3/LRFN4 coated plates (2.5 μg/mL, 100 μL/well). approximately 50%-70% will adhere after 30 minutes at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

5445-SA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SALM3/LRFN4

Synaptic adhesion-like molecule 3 (SALM3; also leucine-rich repeat and fibronectin type-III domain-containing protein 4 (Lrfn4) is an approximately 90 kDa member of the Lrfn family of type I transmembrane glycoproteins (1).  Human SALM3 is synthesized as a 635 amino acid (aa) precursor that contains a 16 aa signal sequence, a 502 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 96 aa cytoplasmic region.  The ECD consists of seven leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1-2).  In addition, there are six potential sites for N-linked glycosylation. The C-terminal region contains an intracellular PDZ binding domain, which is conserved among SALMs 1-3, but is absent in SALMs 4 and 5 (3). Mature human SALM3 shares 97% aa sequence identity with mature mouse SALM3.  Northern blot analysis showed that in mice, SALM3 is strongly expressed in the adult brain and is also present in the adult testis (1). It is distributed throughout the neuron, including the growth cone (3).  In the developing mouse embryo, a temporal expression profile blot revealed a general increment of expression around E10.5, with weak expression detected before E10.5 (1). SALM3, like the other SALMs, promotes neurite outgrowth (3).  Specifically, the SALMs modify total outgrowth and neurite branching (3).  SALM3 may also be involved in synapse formation, synaptic maintenance, and other cellular interactions (3).

References

  1. Morimura, N. et al. (2006) Gene 380:72.
  2. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
  3. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.

Long Name

Synaptic Adhesion-like Molecule 3/Leucine-rich Repeat and Fibronectin Type III Domain Containing 4

Alternate Names

FIGLER6, LRFN4

Entrez Gene IDs

78999 (Human); 225875 (Mouse); 688721 (Rat)

Gene Symbol

LRFN4

UniProt

Q6PJG9 (Human)

Additional SALM3/LRFN4 Products

Product Documents for Recombinant Human SALM3 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human SALM3 Protein, CF

For research use only

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