Recombinant Human Semaphorin 3B Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9518-S3

R&D Systems, part of Bio-Techne
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9518-S3-025
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Key Product Details

Source

T. ni (baculovirus)

Accession #

Q13214-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Semaphorin 3B Proteins and Enzymes »

Product Specifications

Source

Trichoplusia ni, T. ni (baculovirus)-derived human Semaphorin 3B protein
Human Semaphorin 3B
(Ala25-Gly727(Arg551Ala, Arg554Ala))
Accession # Q13214-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala25

Predicted Molecular Mass

105 kDa

SDS-PAGE

96-116 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Semaphorin 3B Fc Chimera is coated at 1 μg/mL, 100 μL/well, it binds to Recombinant Rat Neuropilin‑1 Fc Chimera (Catalog # 566-N1) with an ED50 of 25-150 ng/mL.

Scientific Data Images for Recombinant Human Semaphorin 3B Fc Chimera Protein, CF

Recombinant Human Semaphorin 3B Fc Chimera Protein Bioactivity

Recombinant Human Semaphorin 3B Fc Chimera Protein Bioactivity

When Recombinant Human Semaphorin 3B Fc Chimera (Catalog # 9618-S3) is coated at 1 µg/mL, 100 µL/well, it binds to Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) with an ED50 of 25-150 ng/mL.

Formulation, Preparation and Storage

9518-S3
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid, NaCl and Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 3B

Semaphorin 3B (Sema3B), also known as Semaphorin V and Sema A(V), is an approximately 90 kDa member of the semaphorin family that plays a role in cancer, neuronal and vascular development (1-4). Class 3 semaphorins are secreted molecules found in vertebrates and contain a common domain structure. The Sema3B domains consist of a 484 aa Sema domain, followed by a plexin-semaphorin-integrin (PSI) domain, an 87 aa Ig-like C2-type domain, and a 21 aa basic domain (5). Within these domains, human Sema3B shares 88% and 90% aa sequence identity with mouse and rat Sema3B, respectively. Like other Class 3 semaphorins, Sema3B signaling is transduced by a transmembrane plexin-A dimer, which also has a Sema domain and is coupled to kinase pathways (6, 7). Sema3B binds indirectly to plexins, and requires interaction with neuropilins for activity (6, 7). Repulsive signals through Neuropilin-1 (Npn-1) and attractive signals through Neuropilin-2 (Npn-2) have been identified, affecting adhesion molecule activity in opposite directions (6, 8). In tumors, Sema3B competes with VEGF165 for binding to Npn-1, which in part down-regulates tumor angiogenesis, inhibits tumor cell proliferation and promotes apoptosis (9, 10). However, it is also proposed to promote dissemination and metastatic progression by stimulating Npn-1 mediated IL-8 secretion in a subset of tumors that over-express Sema3B (11). In differentiating osteoblasts, Sema3B expression is induced by vitamin D and promotes osteoclastogenesis (12).

References

  1. Sekido, Y. et al. (1996) Proc Natl Acad Sci U S A. 93:4120.
  2. Gu, C. and E. Giraudo. (2013) Exp. Cell Res. 319:1306.
  3. Sabag, A. et al. (2012) PLoS One. 7:e42912.
  4. Aghajanian, H. et al. (2014) J. Biol. Chem. 289:17971.
  5. Yazdani, U. and J. Terman. (2006) Genome Biology. 7:211.
  6. Halloran, M. C. and M. A. Wolman (2006) Curr. Opin. Cell Biol. 18:533.
  7. Neufeld, G. and O. Kessler (2008) Nat. Rev. Cancer 8:632.
  8. Falk, J. et al. (2005) Neuron 48:63.
  9. Castro-Rivera, E. et al. (2004) Proc. Natl. Acad. Sci. USA 101:11432.
  10. Koyama, N. et al. (2008) Oncogene 27:6581.
  11. Rolny, C. et al. (2008) J. Exp. Med. 205:1155.
  12. Sutton, A. L. M. et al. (2008) Mol. Endocrinol. 22:1370.

Alternate Names

LUCA-1, SEMA3B, SEMA5, SEMAA, SEMAV

Entrez Gene IDs

7869 (Human); 20347 (Mouse); 363142 (Rat)

Gene Symbol

SEMA3B

UniProt

Q13214-1

Additional Semaphorin 3B Products

Product Documents for Recombinant Human Semaphorin 3B Fc Chimera Protein, CF

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