Recombinant Human Semaphorin 4C Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6125-S4

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q9C0C4

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Semaphorin 4C Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Semaphorin 4C protein
Human Semaphorin 4C
(Ala21-Gly663)
Accession # Q9C0C4		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>85%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21

Predicted Molecular Mass

99 kDa

SDS-PAGE

102-123 kDa, reducing conditions

Activity

Measured by its ability to inhibit survival of SK-OV-3 human ovarian carcinoma cells.
The ED50 for this effect is 1-5 μg/mL.

Formulation, Preparation and Storage

6125-S4
Formulation Lyophilized from a 0.2 μm filtered solution in Citric acid, NaCl and Trehalose.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4C

Semaphorin 4C (Sema4C; also Sema I and M­SemaF) is a member of the class IV semaphorin family of type 1 transmembrane glycoproteins (1). Semaphorin 4C signaling is involved in cell attraction and repulsion as well as neural tube closure, stem cell proliferation, and kidney development. Mature Semaphorin 4C consists of a 643 amino acid (aa) extracellular region, a 21 aa transmembrane domain, and a 149 aa PDZ-containing cytoplasmic tail (2-4). The extracellular domain of mature human Semaphorin 4C shares 85% and 84% aa sequence identity with mouse and rat Semaphorin 4C, respectively, and is characterized by a Sema domain, a cysteine rich PSI domain, and an Ig­like C2 ­type domain. Semaphorin 4C is widely expressed during embryogenesis with high expression within the neural tube (5-7). Comparison of Plexin B2-/- and Sema4C-/- mice revealed similar phenotypes, including neural tube closure defects and neonatal lethality. Viable Sema4C-/- neonates have an abnormally developed cerebellum and defects in ventral skin pigmentation (8). Semaphorin 4C has been shown to induce cerebellar granule cell precursor migration and neural stem cell proliferation in in vitro and ex vivo assays, respectively (9). Involvement of Semaphorin 4C in neural stem cell proliferation is further supported by the down-regulation of its expression in neuroblasts during differentiation and up-regulation in proliferating Nestin-postive cells following ischemia (2). Semaphorin 4C is a high affinity ligand for Plexin B2. Upon activation by Sema4C, Plexin B2 can phosphorylate Tyr 1248 on the receptor tyrosine kinase, ErbB2, which then activates RhoA to regulate cytoskeletal dynamics as well as neuronal migration and proliferation (9, 10). During embryogenesis, Semaphorin 4C-Plexin B2 signaling also stimulates branching of the ureteric epithelium in the kidney (11). In adult tissues, the expression pattern of Semaphorin 4C in non-neuronal tissues indicates that Semaphorin 4C-Plexin B2 signaling may additionally regulate vascular and endocrine function (12). Similar to Semaphorin 3A (13, 14), R&D Systems’ in‑house testing data indicate that Semaphorin 4C can inhibit cell survival.

References

  1. Tessier-Lavigne, M. and C.S. Goodman (1996) Science 274:1123.
  2. Wu, H. et al. (2009) J. Mol. Neurosci. 39:27.
  3. Wang, L. et al. (1999) J. Biol. Chem. 274:14137.
  4. Inagaki, S. et al. (1995) FEBS Lett. 370:269.
  5. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
  6. Perala, N. et al. (2010) Dev. Dyn. 239:2722.
  7. Friedel, R.H. et al. (2007) J. Neurosci. 27:3921.
  8. Maier, V. et al. (2011) Mol. Cell. Neurosci. 46:419.
  9. Deng, S. et al. (2007) J. Neurosci. 27:6333.
  10. Swiercz, J.M. et al. (2004) J. Cell. Biol. 165:869.
  11. Perala, N. et al. (2011) Differentiation 81:81.
  12. Zielonka, M. et al. (2010) Exp. Cell Res. 316:2477.
  13. Shirvan, A. et al. (1999) J. Neurochem. 73:961.
  14. Guttman-Raviv, N. et al. (2007) J. Biol. Chem. 282:26294.

Alternate Names

SEMA4C, SEMACL1, Semaf, SEMAI

Entrez Gene IDs

54910 (Human); 20353 (Mouse); 301346 (Rat)

Gene Symbol

SEMA4C

UniProt

Q9C0C4 (Human)

Additional Semaphorin 4C Products

Product Documents for Recombinant Human Semaphorin 4C Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Semaphorin 4C Fc Chimera Protein, CF

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