Recombinant Human Serpin A3 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1295-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Serpin A3/alpha 1-Antichymotrypsin protein
Asn26-Ala423, with a C-terminal 10-His tag
Asn26-Ala423, with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asn26
Predicted Molecular Mass
46 kDa
SDS-PAGE
60-65 kDa, reducing conditions
Activity
Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <8 nM, as measured under the described conditions.
The IC50 value is <8 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1295-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM HEPES and 150 mM NaCl, pH 7.8. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Serpin A3/alpha 1-Antichymotrypsin
Serpin A3 is a member of the Serpin superfamily of the serine protease inhibitors (1). It is synthesized primarily in the liver and secreted as one of the most abundant serpins in plasma (2). It is known to inhibit several serine proteases including chymotrypsin, cathepsin G, chymase, kallikrein 3/prostate specific antigen, and unidentified ectoenzymes that process pro-macrophage stimulating protein (1‑5). Serpin A3 is a major constituent of the plaques associated with Alzheimer’s disease and an inhibitor of amyloid beta peptide degradation (1‑6). Deficiency in Serpin A3 activity due to a point mutation (Leu55Pro) is associated with chronic obstructive pulmonary disease (7). Human Serpin A3 is synthesized as a 423 amino acid precursor (8, 9). The mature protein is secreted and has two forms that differ in their N‑termini (10). One of the forms starting at Asn26 was expressed and purified.
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Kalsheker, N. et al. (2002) Biochem. Soc. Trans. 30:93.
- Duranto, J. et al. (1998) Biochemistry 37:11239.
- Hsieh, M.-C. and B.S. Cooperman (2002) Biochemistry 41:2990.
- Skeel, A. and E.J. Leonard (2001) J. Biol. Chem. 276:21932.
- Abraham, C.R. et al. (2000) Ann. N. Y. Acad. Sci. 920:245.
- Gooptu, B. et al. (2000) Proc. Natl. Acad. Sci. USA 97:67.
- Chandra, T. et al. (1983) Biochemistry 22:5055.
- Morii, M. and J. Travis (1983) J. Biol. Chem. 258:12749.
- Lindmark, B. et al. (1989) Biochim. Biophys. Acta 997:90.
Alternate Names
alpha 1-Antichymotrypsin
Entrez Gene IDs
12 (Human)
Gene Symbol
SERPINA3
UniProt
Additional Serpin A3/alpha 1-Antichymotrypsin Products
Product Documents for Recombinant Human Serpin A3 Protein, CF
Product Specific Notices for Recombinant Human Serpin A3 Protein, CF
For research use only
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