Recombinant Human Serpin B4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9437-PI

R&D Systems, part of Bio-Techne
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9437-PI-050
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P48594

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Serpin B4 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Serpin B4 protein
Asn2-Pro390
with an N-terminal Met and 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

46 kDa

SDS-PAGE

38-44 kDa, reducing conditions

Activity

Measured by its ability to inhibit chymase cleavage of a fluorogenic peptide substrate, Suc-AAPF-AMC.
The IC50 is <20 nM, as measured under the described conditions.

Formulation, Preparation and Storage

9437-PI
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution
Reconstitute at 0.5 mg/mL in deionized water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin B4

Serpin B4, also known as squamous cell carcinoma antigen 2 (SCCA-2), is an approximately 45 kDa member of the serpin superfamily of serine protease inhibitors (1). Serpin B4 belongs to the subgroup ovalbumin-related serpins which are involved in the intracellular regulation of apoptosis, inflammation, angiogenesis and embryogenesis (2). Serpin B4 shares 91% sequence identity to Serpin B3, also known as squamous cell carcinoma antigen 1 (SCCA-1), with key differences in the reactive site loops leading to inhibition of distinct classes of proteases (3). Serpin B4 inhibits chymotrypsin-like serine proteases (3). Serpin B4 inhibits apoptosis via STAT activation in an infection (4) and in carcinoma cells (5). It is a mediator in Ras-driven cancer and inflammation (6). Serpin B4 also is a core protein from which Pso p27, an autoantigen present in chronic inflammatory diseases, is derived (7, 8).

References

  1. Law, R. et al. (2006) Genome Biol. 7:216.
  2. Benarafa, C. and Remold-O'Donnell, E. (2005) Proc. Natl. Acad. Sci. 102:11367.
  3. Schick, C. et al. (1998) Proc. Natl. Acad. Sci. 95:13465.
  4. Song, K.J. et al. (2012) Korean J. Parasitol. 50:1.
  5. Ahmed, S.T. and Darnell, J.E. (2009) Biochem. Biophys. Res. Commun. 378:821.
  6. Catanzaro, J.M. et al. (2014) Cell Cycle 13:3155.
  7. Lysvand, H. et al. (2014) Biochim. Biophys. Acta. 1842:734.
  8. Iverson, O.J. et al. (2017) Clin. Immunol. 174:10.

Long Name

Serpin Family B Member 4

Alternate Names

LEUPIN, Peptidase Inhibitor 11, PI11, SCCA2, SerpinB4

Entrez Gene IDs

6318 (Human); 20248 (Mouse); 498209 (Rat)

Gene Symbol

SERPINB4

UniProt

P48594

Additional Serpin B4 Products

Product Documents for Recombinant Human Serpin B4 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Serpin B4 Protein, CF

For research use only

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