Recombinant Human Serpin C1/Antithrombin-III Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1267-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Serpin C1/Antithrombin-III protein
His33-Lys464, with a C-terminal 10-His tag
His33-Lys464, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His33
Predicted Molecular Mass
50 kDa
SDS-PAGE
55-65 kDa, reducing conditions
Formulation, Preparation and Storage
1267-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl, pH 6.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Serpin C1/Antithrombin-III
Serpin C1 is a member of the Serpin superfamily of the serine protease inhibitors (1). It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa (2). Similar to Serpins A5 and D1, its thrombin inhibitory activity is enhanced by heparin. Hereditary and acquired Serpin C1 deficiency is the cause of an increased thrombotic tendency in many cases (3). For example, acquired Serpin C1 deficiency is a common condition in sepsis, after major trauma or surgery (4).
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Chuang, Y.-J. et al. (2001) Biochemistry 40:6670.
- Vinazzer, H. (1999) Semin. Thromb. Hemost. 25:257.
- Risberg, B. (1998) Blood Coagul. Fibrinolysis Suppl. 3:S3.
Alternate Names
Antithrombin-III, ATIII
Gene Symbol
SERPINC1
UniProt
Additional Serpin C1/Antithrombin-III Products
Product Documents for Recombinant Human Serpin C1/Antithrombin-III Protein, CF
Product Specific Notices for Recombinant Human Serpin C1/Antithrombin-III Protein, CF
For research use only
Loading...
Loading...
Loading...
Loading...