Recombinant Human Serpin E1/PAI-1 Protein, CF Best Seller
R&D Systems, part of Bio-Techne | Catalog # 1786-PI
Key Product Details
Source
Sf 21 (stably transfected)
Accession #
Conjugate
Unconjugated
Applications
Inhibition Activity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived human Serpin E1/PAI-1 protein
Met1-Pro402, with a C-terminal 10-His tag
Met1-Pro402, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser22 & Val24
Predicted Molecular Mass
44 kDa
SDS-PAGE
43 kDa, reducing conditions
Activity
Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC).
The IC50 value is <13 nM, as measured under the described conditions.
The IC50 value is <13 nM, as measured under the described conditions.
Reviewed Applications
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Scientific Data Images for Recombinant Human Serpin E1/PAI-1 Protein, CF
Recombinant Human Serpin E1/PAI-1 Protein Enzyme Activity
Recombinant Human Serpin E1/PAI-1 (Catalog # 1786-PI) is measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC).Formulation, Preparation and Storage
1786-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, NaCl and CHAPS. |
| Reconstitution | Reconstitute at 500 μg/mL in sterile 50 mM Sodium Acetate and 100 mM NaCl, pH 5.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Serpin E1/PAI-1
As a member of the Serpin superfamily of serine protease inhibitors, Serpin E1/PAI‑1 is the principal inhibitor of urokinase‑type plasminogen activator (uPA) and tissue‑type PA (1, 2). As important regulators of extracellular matrix remodeling, uPA and PAI‑1 play a major role in many processes such as angiogenesis, tumor invasion and obesity (2‑4). For example, uPA and PAI-1 are the only tumor prognostic factors validated at the highest level of evidence with regard to their clinical utility in breast cancer (5). The human PAI-1 is initially synthesized as 402 amino acid precursor with a N‑terminal signal peptide (6, 7). PAI‑1 may exist in one of two possible conformations, designated as active or latent (8). The purified rhPAI‑1 is active against rhuPA. The heterogeneity at the N‑terminus of the purified
rhPAI‑1 has been observed before for both the recombinant and native proteins (9).
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Stefansson, S. et al. (2003) Curr. Pharm. Des. 9:1545.
- Duffy, M.J. (2002) Clin. Chem. 48:1194.
- Juhan-Vague, I. et al. (2003) J. Thromb. Haemost. 1:1575.
- Harbeck, N. et al. (2002) Clin. Breast Cancer 3:196.
- Pannekoek, H. et al. (1986) EMBO J. 5:2539.
- Ginsburg, D. et al. (1986) J. Clin. Invest. 78:1673.
- Wang, Z. et al. (1996) Biochemistry 35:16443.
- Stromqvist, M. et al. (1994) Protein Expr. Purif. 5:309.
Long Name
Plasminogen Activator Inhibitor
Alternate Names
Nexin, PAI-1, PAI1, PLANH1
Gene Symbol
SERPINE1
UniProt
Additional Serpin E1/PAI-1 Products
Product Documents for Recombinant Human Serpin E1/PAI-1 Protein, CF
Product Specific Notices for Recombinant Human Serpin E1/PAI-1 Protein, CF
For research use only
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