Recombinant Human sFRP-2 Protein

R&D Systems, part of Bio-Techne | Catalog # 6838-FR

R&D Systems, part of Bio-Techne
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Carrier Free
6838-FR-025/CF
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With Carrier
6838-FR-025
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Key Product Details

Source

NS0

Accession #

AAH08666

Conjugate

Unconjugated

Applications

Bioactivity

View all sFRP-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human sFRP-2 protein
Leu25-Cys295

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu25 (major) & Phe26 (minor)

Predicted Molecular Mass

31 kDa

SDS-PAGE

34-36 kDa, reducing conditions

Activity

Measured by its ability to synergize with Wnt-3a to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
The ED50 for this effect is 10-40 ng/mL in the presence of 20 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 6838-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 6838-FR/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: sFRP-2

Secreted Frizzled Related Protein‑2 (sFRP‑2) belongs to a family of Wnt‑binding proteins with homology to the ligand‑binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30‑35 kDa in size and contain an N-terminal Frizzled‑like domain with 10 conserved cysteines and a Netrin‑like C‑terminal domain (1, 2). Mature human sFRP‑2, also known as SARP‑1, SDF‑5, and FRP‑2, shares 99% aa sequence identity with mouse and rat sFRP‑2 (3). sFRP‑2 is widely expressed during embryogenesis and in the adult tissues including the eye, heart, lung, colon, intestine, smooth muscle, pancreas, prostate, testis, kidney, brain, teeth and joints, craniofacial mesenchyme, and preadipocytes (3‑6). Depending on the context, sFRP‑2 can exert either positive or negative effects on Wnt signaling (7‑9). It also inhibits BMP‑induced effects (8, 10). sFRP‑2 can be incorporated into the extracellular matrix through interactions with Fibronectin and Integrin alpha5 beta1 (11). sFRP‑2 plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation (8, 10, 12, 13). sFRP‑2 is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity (10, 14). At physiological concentrations sFRP‑2 enhances BMP‑1 mediated proteolysis of Pro‑Collagen I, whereas at higher concentrations it inhibits BMP‑1 activity (15, 16). This difference is significant considering that sFRP‑2 is up‑regulated in fibrotic areas of the heart following myocardial infarction (15, 16). Elevated levels of sFRP‑2 then promote the recovery of cardiac function by reducing collagen deposition, remodeling, and calcification and by promoting the engraftment of mesenchymal stem cells into the heart (8, 15).

References

  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Hu, E. et al. (1998) Biochem. Biophys. Res. Commun. 247:287.
  4. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
  5. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
  6. Leimeister, C. et al. (1998) Mech. Dev. 75:29.
  7. Galli, L.M. et al. (2006) Dev. Dyn. 235:681.
  8. Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645.
  9. von Marschall, Z. and L.W. Fisher (2010) Biochem. Biophys. Res. Commun. 400:299.
  10. Oshima, T. et al. (2005) Blood 106:3160.
  11. Lee, J.-L. et al. (2004) J. Biol. Chem. 279:14602.
  12. Sugiyama, Y. et al. (2010) Dev. Biol. 338:193.
  13. Descamps, S. et al. (2008) Cell Tissue Res. 332:299.
  14. Roth, W. et al. (2000) Oncogene 19:4210.
  15. He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110.
  16. Kobayashi, K. et al. (2009) Nat. Cell Biol. 11:46.

Long Name

Secreted Frizzled Related Protein 2

Alternate Names

SARP-1, SDF-5, sFRP2

Entrez Gene IDs

6423 (Human); 20319 (Mouse)

Gene Symbol

SFRP2

UniProt

AAH08666

Additional sFRP-2 Products

Product Documents for Recombinant Human sFRP-2 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 6838-FR (with carrier)
Download SDS 6838-FR/CF (carrier free)

Product Specific Notices for Recombinant Human sFRP-2 Protein

For research use only

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