Recombinant Human sFRP-4 Protein
R&D Systems, part of Bio-Techne | Catalog # 1827-SF
Key Product Details
Source
NS0
Accession #
Structure / Form
Recombinant Human sFRP-4 is prone to proteolytic cleavage at C-terminus, therefore the poly-His tag at C-terminus may not be present in the preparation.
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human sFRP-4 protein
Ala22-Val346, with a C-terminal 10-His tag
Ala22-Val346, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala22
Predicted Molecular Mass
38.9 kDa
SDS-PAGE
55 kDa, reducing conditions
Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is 4-20 µg/mL, in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Optimal dilutions should be determined by each laboratory for each application.
The ED50 for this effect is 4-20 µg/mL, in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Optimal dilutions should be determined by each laboratory for each application.
Reviewed Applications
Read 1 review rated 5 using 1827-SF in the following applications:
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1827-SF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 1827-SF/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: sFRP-4
Secreted Frizzled Related Proteins (sFRPs) are a family of vertebrate proteins which contain homology to the ligand-binding domain of the Frizzled family of transmembrane receptors. sFRPs are approximately 30-35 kDa in size and are comprised of 3 domains: a signal sequence; a cysteine-rich domain (CRD) of about 110 amino acids (aa) with high degree of similarity to the Frizzled proteins, including 10 conserved cysteines; and a 175 aa conserved hydrophilic carboxy terminal region. Because sFRPs contain a CRD very similar to the region responsible for binding Wnt ligands in Frizzleds, sFRPs are thought to act as soluble antagonists of Wnt signals, often postulated to act as tumor suppressor genes (1).
sFRP-4, also known as DDC-4, FrpAP, frpHE and FrzB-2, is expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1, 2). This protein shows complex functions with respect to cell survival: it is up‑regulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is up‑regulated in endometrial and breast carcinomas (6, 7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, this suggest that its role in cancer is likely to be tissue dependent (6). In addition sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with osteomalacia that antagonizes renal Wnt signaling (7). Of all the secreted frizzled related proteins, sFRP-4 is most closely related to sFRP-3 (1). Mouse and human sFRP-4 proteins share 92% aa identity.
References
- Jones, S.E. and C. Jomary (2002) Bioessays 24:811.
- Wolf, V. et al. (1997) FEBS Letters 417:385.
- Drake, J.M. et al. (2003) Apoptosis 8:389.
- James, I.E. et al. (2000) Osteoarthritis & Cartilage 8:452.
- Lacher, M.D. et al. (2003) Cell Death Differ. 10:528.
- Abu-Jawdeh, G. et al. (1999) Lab Investigation 79:439.
- Wong, S.C.C. et al. (2002) J. Pathology 196:145.
- Berndt, T. et al. (2003) J. Clin. Invest. 112:785.
Long Name
Secreted Frizzled Related Protein 4
Alternate Names
DDC-4, FRP-AP, frpHE, FrzB2, sFRP4
Gene Symbol
SFRP4
UniProt
Additional sFRP-4 Products
Product Documents for Recombinant Human sFRP-4 Protein
Product Specific Notices for Recombinant Human sFRP-4 Protein
For research use only
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